Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-0349-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD34 Monoclonal Antibody (4H11), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family which also includes endoglycan and podocalyxin. Members of this family are single pass transmembrane proteins with a heavily glycosylated extracellular and N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors, and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines, and by vascular endothelia in the adult. Epitopes of CD34 have been assigned to three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage by neuraminidase, chymopapain, or O-glycoprotease. According to this analysis, the 4H11 antibody belongs to class III, indicating that it reacts with a protein epitope. Applications Reported: This 4H11 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB). The 4H11 monoclonal antibody can also be used to inhibit proliferation and induce apoptosis in CD34+ cell lines including MOLM-9, JURL-MK1 and HEL. Applications Tested: This 4H11 antibody has been tested by flow cytometric analysis of TF-1 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4H11
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.
A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1.
Combined inhibition of β-catenin and Bcr-Abl synergistically targets tyrosine kinase inhibitor-resistant blast crisis chronic myeloid leukemia blasts and progenitors in vitro and in vivo.
Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats.
Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.
Human Induced Pluripotent Stem Cell-Derived Macrophages Share Ontogeny with MYB-Independent Tissue-Resident Macrophages.
Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.
A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.
A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis.
Microvesicles from Mesenchymal Stromal Cells Are Involved in HPC-Microenvironment Crosstalk in Myelodysplastic Patients.
Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.
Differences in the Osteogenic Differentiation Capacity of Omental Adipose-Derived Stem Cells in Obese Patients With and Without Metabolic Syndrome.
Single-cell mass cytometry reveals intracellular survival/proliferative signaling in FLT3-ITD-mutated AML stem/progenitor cells.
JAK2/STAT5 inhibition by nilotinib with ruxolitinib contributes to the elimination of CML CD34+ cells in vitro and in vivo.
Colony-stimulating factor-1 mediates macrophage-related neural damage in a model for Charcot-Marie-Tooth disease type 1X.
Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines.
Binding of L-selectin to the vascular sialomucin CD34.
Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34.
Fan C, Ji Q, Zhang C, Xu S, Sun H, Li Z
Molecular medicine reports 2019 Oct;20(4):3123-3130
Molecular medicine reports 2019 Oct;20(4):3123-3130
A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1.
Böiers C, Richardson SE, Laycock E, Zriwil A, Turati VA, Brown J, Wray JP, Wang D, James C, Herrero J, Sitnicka E, Karlsson S, Smith AJH, Jacobsen SEW, Enver T
Developmental cell 2018 Feb 5;44(3):362-377.e7
Developmental cell 2018 Feb 5;44(3):362-377.e7
Combined inhibition of β-catenin and Bcr-Abl synergistically targets tyrosine kinase inhibitor-resistant blast crisis chronic myeloid leukemia blasts and progenitors in vitro and in vivo.
Zhou H, Mak PY, Mu H, Mak DH, Zeng Z, Cortes J, Liu Q, Andreeff M, Carter BZ
Leukemia 2017 Oct;31(10):2065-2074
Leukemia 2017 Oct;31(10):2065-2074
Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats.
Yang H, Wu S, Feng R, Huang J, Liu L, Liu F, Chen Y
Stem cell research & therapy 2017 Nov 21;8(1):267
Stem cell research & therapy 2017 Nov 21;8(1):267
Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.
Li J, Mao Q, He J, She H, Zhang Z, Yin C
Stem cell research & therapy 2017 Mar 9;8(1):55
Stem cell research & therapy 2017 Mar 9;8(1):55
Human Induced Pluripotent Stem Cell-Derived Macrophages Share Ontogeny with MYB-Independent Tissue-Resident Macrophages.
Buchrieser J, James W, Moore MD
Stem cell reports 2017 Feb 14;8(2):334-345
Stem cell reports 2017 Feb 14;8(2):334-345
Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.
Yang CT, Ma R, Axton RA, Jackson M, Taylor AH, Fidanza A, Marenah L, Frayne J, Mountford JC, Forrester LM
Stem cells (Dayton, Ohio) 2017 Apr;35(4):886-897
Stem cells (Dayton, Ohio) 2017 Apr;35(4):886-897
A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.
Zhu N, Wang H, Wang B, Wei J, Shan W, Feng J, Huang H
Stem cells international 2016;2016:5687589
Stem cells international 2016;2016:5687589
A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis.
Juneja SC, Viswanathan S, Ganguly M, Veillette C
Bone marrow research 2016;2016:3152065
Bone marrow research 2016;2016:3152065
Microvesicles from Mesenchymal Stromal Cells Are Involved in HPC-Microenvironment Crosstalk in Myelodysplastic Patients.
Muntión S, Ramos TL, Diez-Campelo M, Rosón B, Sánchez-Abarca LI, Misiewicz-Krzeminska I, Preciado S, Sarasquete ME, de Las Rivas J, González M, Sánchez-Guijo F, Del Cañizo MC
PloS one 2016;11(2):e0146722
PloS one 2016;11(2):e0146722
Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.
Jackson M, Ma R, Taylor AH, Axton RA, Easterbrook J, Kydonaki M, Olivier E, Marenah L, Stanley EG, Elefanty AG, Mountford JC, Forrester LM
Stem cells translational medicine 2016 Aug;5(8):981-90
Stem cells translational medicine 2016 Aug;5(8):981-90
Differences in the Osteogenic Differentiation Capacity of Omental Adipose-Derived Stem Cells in Obese Patients With and Without Metabolic Syndrome.
Oliva-Olivera W, Leiva Gea A, Lhamyani S, Coín-Aragüez L, Alcaide Torres J, Bernal-López MR, García-Luna PP, Morales Conde S, Fernández-Veledo S, El Bekay R, Tinahones FJ
Endocrinology 2015 Dec;156(12):4492-501
Endocrinology 2015 Dec;156(12):4492-501
Single-cell mass cytometry reveals intracellular survival/proliferative signaling in FLT3-ITD-mutated AML stem/progenitor cells.
Han L, Qiu P, Zeng Z, Jorgensen JL, Mak DH, Burks JK, Schober W, McQueen TJ, Cortes J, Tanner SD, Roboz GJ, Kantarjian HM, Kornblau SM, Guzman ML, Andreeff M, Konopleva M
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2015 Apr;87(4):346-56
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2015 Apr;87(4):346-56
JAK2/STAT5 inhibition by nilotinib with ruxolitinib contributes to the elimination of CML CD34+ cells in vitro and in vivo.
Gallipoli P, Cook A, Rhodes S, Hopcroft L, Wheadon H, Whetton AD, Jørgensen HG, Bhatia R, Holyoake TL
Blood 2014 Aug 28;124(9):1492-501
Blood 2014 Aug 28;124(9):1492-501
Colony-stimulating factor-1 mediates macrophage-related neural damage in a model for Charcot-Marie-Tooth disease type 1X.
Groh J, Weis J, Zieger H, Stanley ER, Heuer H, Martini R
Brain : a journal of neurology 2012 Jan;135(Pt 1):88-104
Brain : a journal of neurology 2012 Jan;135(Pt 1):88-104
Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines.
Elknerová K, Lacinová Z, Soucek J, Marinov I, Stöckbauer P
Neoplasma 2007;54(4):311-20
Neoplasma 2007;54(4):311-20
Binding of L-selectin to the vascular sialomucin CD34.
Baumheter S, Singer MS, Henzel W, Hemmerich S, Renz M, Rosen SD, Lasky LA
Science (New York, N.Y.) 1993 Oct 15;262(5132):436-8
Science (New York, N.Y.) 1993 Oct 15;262(5132):436-8
Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34.
Sutherland DR, Watt SM, Dowden G, Karhi K, Baker MA, Greaves MF, Smart JE
Leukemia 1988 Dec;2(12):793-803
Leukemia 1988 Dec;2(12):793-803
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of TF-1 cell line with 0.25 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD34 Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Total viable cells were used for analysis.