PA5-48233

antibody from Invitrogen Antibodies

Targeting: HBB beta-globin, CD113t-C

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-48233

Provider

Invitrogen Antibodies

Product name

Anti-HBB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

Lot Dependent

Storage

-20° C, Avoid Freeze/Thaw Cycles

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of HBB in formalin fixed and paraffin embedded human liver tissue. Samples were probed with a HBB Antibody (C-term) (Product # PA5-48233) followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HBB in K562 cells (right histogram) compared to a negative control cell (left histogram). Samples were incubated with a HBB Antibody (C-term) (Product # PA5-48233) followed by incubation with a FITC-conjugated goat-anti-rabbit secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HBB in K562 cells. Cells were fixed, permeabilized, and stained with a HBB polyclonal antibody (Product # PA5-48233, green line) at a dilution of 1:25 in 2% BSA. After the incubation of the primary antibody for 1 hour at 37°C, the cells were stained with an Alexa Fluor® 488 goat anti-rabbit lgG (H+L) antibody at 1:400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.