TA800916

antibody from OriGene

Targeting: CD99 MIC2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA800916

Provider

OriGene

Proper citation

OriGene Cat#TA800916, RRID:AB_2625736

Product name

CD99 mouse monoclonal antibody, clone OTI4E1 (formerly 4E1)

Antibody type

Monoclonal

Description

CD99 mouse monoclonal antibody, clone OTI4E1 (formerly 4E1)

Host

Mouse

Conjugate

Unconjugated

Epitope

CD99

Isotype

IgG

Antibody clone number

OTI4E1

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD99 (RC204056, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD99.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800916)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800916)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of living Jurkat cells,using anti-CD99 antibody(TA800916),(Red),compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC