Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB3905P - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CD99 PE-conjugated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CD99 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) PILR-alpha and rmPILR-beta is observed.
- Reactivity
- Mouse
- Host
- Goat
- Antigen sequence
Q8VCN6
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Ectodomain shedding of CD99 within highly conserved regions is mediated by the metalloprotease meprin β and promotes transendothelial cell migration.
Bedau T, Peters F, Prox J, Arnold P, Schmidt F, Finkernagel M, Köllmann S, Wichert R, Otte A, Ohler A, Stirnberg M, Lucius R, Koudelka T, Tholey A, Biasin V, Pietrzik CU, Kwapiszewska G, Becker-Pauly C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Mar;31(3):1226-1237
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Mar;31(3):1226-1237
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD99 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse CD99 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3905P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.