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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- AF3968 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD99 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CD99 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD99 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P14209- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Ectodomain shedding of CD99 within highly conserved regions is mediated by the metalloprotease meprin β and promotes transendothelial cell migration.
Evolutionarily conserved paired immunoglobulin-like receptor α (PILRα) domain mediates its interaction with diverse sialylated ligands.
Bedau T, Peters F, Prox J, Arnold P, Schmidt F, Finkernagel M, Köllmann S, Wichert R, Otte A, Ohler A, Stirnberg M, Lucius R, Koudelka T, Tholey A, Biasin V, Pietrzik CU, Kwapiszewska G, Becker-Pauly C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Mar;31(3):1226-1237
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Mar;31(3):1226-1237
Evolutionarily conserved paired immunoglobulin-like receptor α (PILRα) domain mediates its interaction with diverse sialylated ligands.
Sun Y, Senger K, Baginski TK, Mazloom A, Chinn Y, Pantua H, Hamidzadeh K, Ramani SR, Luis E, Tom I, Sebrell A, Quinones G, Ma Y, Mukhyala K, Sai T, Ding J, Haley B, Shadnia H, Kapadia SB, Gonzalez LC, Hass PE, Zarrin AA
The Journal of biological chemistry 2012 May 4;287(19):15837-50
The Journal of biological chemistry 2012 May 4;287(19):15837-50
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD99 in HepG2 Human Cell Line. CD99 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human CD99 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3968) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD99 in Human CD3+ Lymphocytes by Flow Cytometry. Human whole blood CD3+ lymphocytes were stained with Goat Anti-Human CD99 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3968, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
