PA5-118330

antibody from Invitrogen Antibodies

Targeting: TXNDC5 EndoPDI, ERp46, FLJ21353, FLJ90810, Hcc-2, MGC3178, PDIA15

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118330

Provider

Invitrogen Antibodies

Product name

TXNDC5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of TXNDC5 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with TXNDC5 Polyclonal Antibody (Product # PA5-118330, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of TXNDC5 in Lane A: 0.5 mg HepG2 Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118330) with a dilution of 1:100 , followed by Clean-Blot HRP Detection reagent using a dilution of 1:1,000. Assay was performed under reducing conditions. Predicted band size: 48 kDa , Observed band size : 50 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TXNDC5 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 µL with TXNDC5 Polyclonal Antibody (Product # PA5-118330) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: TXNDC5 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 50 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human TXNDC5 in human tonsil with TXNDC5 Polyclonal Antibody (Product # PA5-118330, 1:5,000 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human TXNDC5 in human stomach with TXNDC5 Polyclonal Antibody (Product # PA5-118330, 1:5,000 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TXNDC5 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 µL with TXNDC5 Polyclonal Antibody (Product # PA5-118330) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: TXNDC5 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 50 kDa.