Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30658 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOHLH2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SOHLH2 Polyclonal Antibody (Product # PA5-30658). Sample (30 µg of whole cell lysate). Lane A: NT2D1. Lane B: MCF-7. 12% SDS PAGE. SOHLH2 Polyclonal Antibody (Product # PA5-30658) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SOHLH2 in paraformaldehyde-fixed MCF-7 cells using a SOHLH2 polyclonal antibody (Product # PA5-30658) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using SOHLH2 (Product # PA5-30658) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.