Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-90725 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-90725, RRID:AB_11009707
- Product name
- Rabbit Polyclonal FRMD6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat FRMD6 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Poor-prognosis colon cancer is defined by a molecularly distinct subtype and develops from serrated precursor lesions.
De Sousa E Melo F, Wang X, Jansen M, Fessler E, Trinh A, de Rooij LP, de Jong JH, de Boer OJ, van Leersum R, Bijlsma MF, Rodermond H, van der Heijden M, van Noesel CJ, Tuynman JB, Dekker E, Markowetz F, Medema JP, Vermeulen L
Nature medicine 2013 May;19(5):614-8
Nature medicine 2013 May;19(5):614-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FRMD6 Antibody [NBP1-90725] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FRMD6 Antibody [NBP1-90725] - Analysis in human cell lines A-431 and HEK293 using anti-FRMD6 antibody. Corresponding FRMD6 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: FRMD6 Antibody [NBP1-90725] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FRMD6 Antibody [NBP1-90725] - Staining of human duodenum shows moderate to strong cyoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FRMD6 Antibody [NBP1-90725] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FRMD6 Antibody [NBP1-90725] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FRMD6 Antibody [NBP1-90725] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: FRMD6 Antibody [NBP1-90725] - Staining of mouse cerebral cortex shows moderate to strong positivity in more than 75 % of neurons in the somatosensory cortex.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: FRMD6 Antibody [NBP1-90725] - Staining of mouse brain shows moderate to strong positivity in more than 75 % of purkinje cells in the cerebellum.