Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX108732 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX108732, RRID:AB_1949958
- Product name
- CD66e antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30?g whole cell lysate)A: 293TB: A431 (GTX27909)C: H1299D: HeLa S3 (GTX14654)E: Hep G2 (GTX27900)F: MOLT4 (GTX27912)G: Raji (GTX27908)7.5% SDS PAGEGTX108732 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CD66e antibody detects CD66e protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with CD66e antibody (GTX108732) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using CD66e(GTX108732) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SG xenograft, using CD66e(GTX108732) antibody at 1:500 dilution.