DM1202

antibody from OriGene

Targeting: CEACAM5 CD66e, CEA

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

DM1202

Provider

OriGene

Product name

CEACAM5 mouse monoclonal antibody, clone 26/3/13, Purified

Antibody type

Monoclonal

Description

CEACAM5 mouse monoclonal antibody, clone 26/3/13, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

CEACAM5

Isotype

IgG

Antibody clone number

26/3/13

Vial size

100 µg

Concentration

2.0 mg/ml

References

Submitted references

Granulocyte CEACAM3 is a phagocytic receptor of the innate immune system that mediates recognition and elimination of human-specific pathogens.

Schmitter T, Agerer F, Peterson L, Munzner P, Hauck CR
The Journal of experimental medicine 2004 Jan 5;199(1):35-46

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Figure 2. FACS analysis of BOSC23 cells using 26/3/13 antibody . BOSC23 cells were transiently transfected with an expres-sion vector encoding either CEACAM5 (red curve) or an irrelevantprotein (control transfectant). Binding of 26/3/13 was detected witha PE conjugated secondary antibody. A positive signal was obtained only with CEACAM5 transfected cells.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Figure 1. Specificity testing of 26/3/13. BOSC23 cells were transiently transfected with expression vectorscontaining either the cDNA of CEACAM1, CEACAM5-8. Recognition of CEACAM3, 4 and of a recombinant transmembrane-anchored PSG1 fusion protein was tested on stably transfected HeLa (CEACAM3, PSG) and CHO cells (CEACAM4), respectively. Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: TET2; PSG: Brified 26/3/13 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM5 expressing cells (red curves).

Validation comment

FC