Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-54625 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CEACAM5/CD66e Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. This antibody recognizes human CEACAM 5. It does not cross-react with CEACAM-1, -3, -4, -6, -7, and -8 or rat CEACAM1, mouse CEACAM1 and mouse CEACAM2.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C in the dark.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Analysis of human granulocyte lysate: Detection using 10 ug/ml Anti-CEACAM5 [5C8C4] followed by HRP coupled secondary pAb. Visualized by Chemiluminescence detection.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Solid phase was coated with 3 ug/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM5/8 [5C8C4] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Human granulocyte lysate: Utilizing 10 ug/ml Anti-CEACAM5/8 [5C8C4]. Detection of the sample using 5 ug/ml pAb Anti-CEA followed by HRP coupled secondary pAb. Visualized by Chemiluminescence detection.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM5 [5C8C4] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).