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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-15070 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CEA Monoclonal Antibody (H.426.3)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- H.426.3
- Vial size
- 100 µL
- Concentration
- 1122 µg/mL
- Storage
- -20°C
Submitted references The role of mucin cell-free DNA detection as a new marker for the study of acellular pseudomyxoma peritonei of appendicular origin by liquid biopsy.
Recurrent intracranial neurenteric cyst with malignant transformation: A case report and literature review.
García-Olmo D, Olmedillas-López S, Cortés-Guiral D, Villarejo P, López Rojo I, Guadalajara H, García Gómez-Heras S, García-Arranz M
Therapeutic advances in medical oncology 2020;12:1758835920928233
Therapeutic advances in medical oncology 2020;12:1758835920928233
Recurrent intracranial neurenteric cyst with malignant transformation: A case report and literature review.
Yang Y, Fang J, Li DA, Wang L, Ji N, Zhang J
Oncology letters 2016 May;11(5):3395-3402
Oncology letters 2016 May;11(5):3395-3402
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CEA/CD66e in extracts from HT29 and MCF7 cells using CEA/CD66e monoclonal antibody (Product # MA5-15070).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CEA Monoclonal Antibody (H.426.3)(Product # MA5-15070) and a 180 kDa band corresponding to Carcinoembryonic antigen-related cell adhesion molecule 5 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), HCT 116 (Lane 2), SW480 (Lane 3), HT-29 (Lane 4), HT-29 treated with IL-6, 100 ng/mL, 48Hrs. (Lane 5), MDA-MB-231 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).Expression of CEACAM5 was found to be higher in MCF-7,HT29 as compared to HCT 116,SW480 and it is upregulated upon treatment with IL-6 in HT 29 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CEA/CD66e in MCF-7 cells using a CEA/CD66e monoclonal antibody (Product # MA5-15070).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CEA/CD66e in paraffin-embedded human colon carcinoma using a CEA/CD66e monoclonal antibody (Product # MA5-15070).
