MA5-13711

antibody from Invitrogen Antibodies

Targeting: CEACAM5 CD66e, CEA

Provider product page for MA5-13711

Western blot

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [13]
Comments [0]
Validations
Immunocytochemistry [1]
Immunohistochemistry [1]
Flow cytometry [1]
Other assay [1]

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Product number: MA5-13711 - Provider product page
Provider: Invitrogen Antibodies
Product name: Anti-CEA Monoclonal Antibody (COL-1), Biotin
Antibody type: Monoclonal
Antigen: Other
Description: MA5-13714 targets CD66e in ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Human samples. The MA5-13714 immunogen is human colon carcinoma extract.
Reactivity: Human, Mouse, Rat
Host: Mouse
Conjugate: Biotin
Isotype: IgG
Antibody clone number: COL-1
Vial size: 500 µL
Concentration: 0.2 mg/ml
Storage: 4° C

CEACAM5 protein structure - MA5-13711 shown in red.

Submitted references Multiple breast cancer cell-lines derived from a single tumor differ in their molecular characteristics and tumorigenic potential.

Mosoyan G, Nagi C, Marukian S, Teixeira A, Simonian A, Resnick-Silverman L, DiFeo A, Johnston D, Reynolds SR, Roses DF, Mosoian A
PloS one 2013;8(1):e55145

Sinonasal tract mucoepidermoid carcinoma: a clinicopathologic and immunophenotypic study of 19 cases combined with a comprehensive review of the literature.

Wolfish EB, Nelson BL, Thompson LD
Head and neck pathology 2012 Jun;6(2):191-207

Mesenchymal stem cells delivered in a microsphere-based engineered skin contribute to cutaneous wound healing and sweat gland repair.

Huang S, Lu G, Wu Y, Jirigala E, Xu Y, Ma K, Fu X
Journal of dermatological science 2012 Apr;66(1):29-36

Primary malignant deciduoid peritoneal mesothelioma: a report of the cytohistological and immunohistochemical appearances.

Ustun H, Astarci HM, Sungu N, Ozdemir A, Ekinci C
Diagnostic cytopathology 2011 Jun;39(6):402-8

Low-grade mucinous adenocarcinoma of the uterine corpus: a rare and deceptively bland form of endometrial carcinoma.

Fujiwara M, Longacre TA
The American journal of surgical pathology 2011 Apr;35(4):537-44

In vitro constitution and in vivo implantation of engineered skin constructs with sweat glands.

Huang S, Xu Y, Wu C, Sha D, Fu X
Biomaterials 2010 Jul;31(21):5520-5

Molecular events associated with epithelial to mesenchymal transition of nasopharyngeal carcinoma cells in the absence of Epstein-Barr virus genome.

Lin JC, Liao SK, Lee EH, Hung MS, Sayion Y, Chen HC, Kang CC, Huang LS, Cherng JM
Journal of biomedical science 2009 Nov 24;16:105

A genetically engineered adenovirus vector targeted to CD40 mediates transduction of canine dendritic cells and promotes antigen-specific immune responses in vivo.

Thacker EE, Nakayama M, Smith BF, Bird RC, Muminova Z, Strong TV, Timares L, Korokhov N, O'Neill AM, de Gruijl TD, Glasgow JN, Tani K, Curiel DT
Vaccine 2009 Nov 23;27(50):7116-24

Antigen expression of human eccrine sweat glands.

Li HH, Zhou G, Fu XB, Zhang L
Journal of cutaneous pathology 2009 Mar;36(3):318-24

Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon.

Tsao TY, Tsai CS, Tung JN, Chen SL, Yue CH, Liao CF, Wang CC, Jiang MC
Molecular and cellular biochemistry 2009 Jul;327(1-2):163-70

Detection of micrometastases and skip metastases with ex vivo sentinel node mapping in carcinoma of the colon and rectum.

Yagci G, Unlu A, Kurt B, Can MF, Kaymakcioglu N, Cetiner S, Tufan T, Sen D
International journal of colorectal disease 2007 Feb;22(2):167-73

Mesenchymal hamartoma of the liver in adulthood: immunohistochemical profiles, clinical and histopathological features in two patients.

Yesim G, Gupse T, Zafer U, Ahmet A
Journal of hepato-biliary-pancreatic surgery 2005;12(6):502-7

Secretory meningiomas.

Colakoğlu N, Demirtaş E, Oktar N, Yüntem N, Islekel S, Ozdamar N
Journal of neuro-oncology 2003 May;62(3):233-41

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CD66e (green) showing staining in the cytoplasm and membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD66e monoclonal antibody (Product # MA5-13714) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human colon carcinoma stained with CEA antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic and luminal surface staining of tumor cells.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD66e in PC12 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD66e monoclonal antibody (Product # MA5-13714) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of CD66e using CD66e Monoclonal Antibody (Product # MA5-13711) on denatured Human LS174T Cells.
Conjugate: Biotin