DM1202

antibody from Acris Antibodies GmbH

Targeting: CEACAM5 CD66e, CEA

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

DM1202

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#DM1202, RRID:AB_977067

Product name

anti CD66e / CEACAM5

Antibody type

Monoclonal

Antigen

Extracted protein of CEACAM5.Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

26/3/13

Vial size

0.1 mg

Concentration

2.0 mg/ml

References

Submitted references

The CEA/CD3-bispecific antibody MEDI-565 (MT111) binds a nonlinear epitope in the full-length but not a short splice variant of CEA.

Peng L, Oberst MD, Huang J, Brohawn P, Morehouse C, Lekstrom K, Baeuerle PA, Wu H, Yao Y, Coats SR, Dall'Acqua W, Damschroder M, Hammond SA
PloS one 2012;7(5):e36412

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Granulocyte CEACAM3 is a phagocytic receptor of the innate immune system that mediates recognition and elimination of human-specific pathogens.

Schmitter T, Agerer F, Peterson L, Munzner P, Hauck CR
The Journal of experimental medicine 2004 Jan 5;199(1):35-46

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Specificity testing of 26/3/13. BOSC23 cells were transiently transfected with expression vectorscontaining either the cDNA of CEACAM1, CEACAM5-8. Recognition of CEACAM3, 4 and of a recombinant transmembrane-anchored PSG1 fusion protein was tested on stably transfected HeLa (CEACAM3, PSG) and CHO cells (CEACAM4), respectively. Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: TET2; PSG: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified 26/3/13 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM5 expressing cells (red curves).

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 2. FACS analysis of BOSC23 cells using 26/3/13 antibody Cat.-No DM1202. BOSC23 cells were transiently transfected with an expres-sion vector encoding either CEACAM5 (red curve) or an irrelevantprotein (control transfectant). Binding of 26/3/13 was detected witha PE conjugated secondary antibody. A positive signal was obtained only with CEACAM5 transfected cells.