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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MA1023 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-CEACAM5/Cd66e Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for CEACAM5/Cd66e detection. Tested with WB, IHC-P in Human. No cross reactivity with other proteins.
- Reactivity
- Human
- Host
- Mouse
- Antibody clone number
- C6G9
- Vial size
- 100μg/vial
- Concentration
- Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of CEACAM5/Cd66e using anti- CEACAM5/Cd66e antibody (MA1023). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Recombinant Human CEACAM5/Cd66e Protein 10ng Lane 2; Recombinant Human CEACAM5/Cd66e Protein 5ng Lane 3: Recombinant Human CEACAM5/Cd66e Protein 2.5ng Lane 4: Recombinant Human CEACAM5/Cd66e Protein 1.25ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- CEACAM5/Cd66e antigen affinity purified monoclonal antibody (Catalog # MA1023) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
- Additional image
