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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-96932 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP Synthase O Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: FAKLVRPPVQ VYGIEGRYAT ALYSAASKQN KLEQVEKELL RVAQILKEPK VAASVLNPYV KRSIKVKSLN DITAKERFSP LTTNLINLLA ENGRLSNTQG VVSAFSTMMS VHRGEVPCTV TSASPLEEAT LSELKTVLKS FLSQGQVLKL EAKTDPSILG GMIVRIGEKY VDMSVKTKIQ KLGRAMREIV; Positive Samples: NCI-H460, A-549, U-251MG, SKOV3, MCF-7, Mouse liver, Mouse lung, Mouse heart, Rat heart; Cellular Location: Mitochondrion, Mitochondrion inner membrane
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.55 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP Synthase O in extracts of various cell lines. Samples were incubated with ATP Synthase O Polyclonal antibody (Product # PA5-96932) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ATP Synthase O was performed in U2OS cells using ATP Synthase O Polyclonal Antibody (Product # PA5-96932).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ATP Synthase O in U2OS cells. Samples were incubated with ATP Synthase O Polyclonal antibody (Product # PA5-96932) using a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ATP Synthase O in paraffin-embedded human thyroid cancer. Samples were incubated with ATP Synthase O Polyclonal antibody (Product # PA5-96932) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ATP Synthase O in paraffin-embedded rat stomach. Samples were incubated with ATP Synthase O Polyclonal antibody (Product # PA5-96932) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
