14569-1-AP

antibody from Proteintech Group

Targeting: BBS5 DKFZp762I194

Provider product page for 14569-1-AP

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [27]
Comments [0]
Validations
Western blot [2]

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Product number: 14569-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14569-1-AP, RRID:AB_10597551
Product name: BBS5 antibody
Antibody type: Polyclonal
Description: KD/KO validated BBS5 antibody (Cat. #14569-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

BBS5 protein structure - 14569-1-AP shown in red.

Submitted references The Immune Checkpoint Protein PD-L1 Regulates Ciliogenesis and Hedgehog Signaling.

Agborbesong E, Li X
Cells 2024 Jun 8;13(12)

The BBSome regulates mitochondria dynamics and function.

Guo DF, Merrill RA, Qian L, Hsu Y, Zhang Q, Lin Z, Thedens DR, Usachev YM, Grumbach I, Sheffield VC, Strack S, Rahmouni K
Molecular metabolism 2023 Jan;67:101654

A program of successive gene expression in mouse one-cell embryos.

Asami M, Lam BYH, Hoffmann M, Suzuki T, Lu X, Yoshida N, Ma MK, Rainbow K, Gužvić M, VerMilyea MD, Yeo GSH, Klein CA, Perry ACF
Cell reports 2023 Feb 28;42(2):112023

The ancestral ESCRT protein TOM1L2 selects ubiquitinated cargoes for retrieval from cilia.

Shinde SR, Mick DU, Aoki E, Rodrigues RB, Gygi SP, Nachury MV
Developmental cell 2023 Apr 24;58(8):677-693.e9

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

Duan S, Li H, Zhang Y, Yang S, Chen Y, Qiu B, Huang C, Wang J, Li J, Zhu X, Yan X
The EMBO journal 2021 Jan 15;40(2):e105499

FBF1 deficiency promotes beiging and healthy expansion of white adipose tissue.

Zhang Y, Hao J, Tarrago MG, Warner GM, Giorgadze N, Wei Q, Huang Y, He K, Chen C, Peclat TR, White TA, Ling K, Tchkonia T, Kirkland JL, Chini EN, Hu J
Cell reports 2021 Aug 3;36(5):109481

A mouse model of BBS identifies developmental and homeostatic effects of BBS5 mutation and identifies novel pituitary abnormalities.

Bentley-Ford MR, Engle SE, Clearman KR, Haycraft CJ, Andersen RS, Croyle MJ, Rains AB, Berbari NF, Yoder BK
Human molecular genetics 2021 Apr 26;30(3-4):234-246

The BBSome assembly is spatially controlled by BBS1 and BBS4 in human cells.

Prasai A, Schmidt Cernohorska M, Ruppova K, Niederlova V, Andelova M, Draber P, Stepanek O, Huranova M
The Journal of biological chemistry 2020 Oct 16;295(42):14279-14290

Genetic interaction of mammalian IFT-A paralogs regulates cilia disassembly, ciliary entry of membrane protein, Hedgehog signaling, and embryogenesis.

Wang W, Allard BA, Pottorf TS, Wang HH, Vivian JL, Tran PV
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 May;34(5):6369-6381

Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons.

Haq N, Schmidt-Hieber C, Sialana FJ, Ciani L, Heller JP, Stewart M, Bentley L, Wells S, Rodenburg RJ, Nolan PM, Forsythe E, Wu MC, Lubec G, Salinas P, Häusser M, Beales PL, Christou-Savina S
PLoS biology 2019 Sep;17(9):e3000414

BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone.

Ye F, Nager AR, Nachury MV
The Journal of cell biology 2018 May 7;217(5):1847-1868

Inhibition of Hedgehog signaling suppresses proliferation and microcyst formation of human Autosomal Dominant Polycystic Kidney Disease cells.

Silva LM, Jacobs DT, Allard BA, Fields TA, Sharma M, Wallace DP, Tran PV
Scientific reports 2018 Mar 21;8(1):4985

DAZ-interacting Protein 1 (Dzip1) Phosphorylation by Polo-like Kinase 1 (Plk1) Regulates the Centriolar Satellite Localization of the BBSome Protein during the Cell Cycle.

Zhang B, Wang G, Xu X, Yang S, Zhuang T, Wang G, Ren H, Cheng SY, Jiang Q, Zhang C
The Journal of biological chemistry 2017 Jan 27;292(4):1351-1360

Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the Atypical MAP Kinase MAPK15 in Caenorhabditis elegans and Human Cells.

Kazatskaya A, Kuhns S, Lambacher NJ, Kennedy JE, Brear AG, McManus GJ, Sengupta P, Blacque OE
Genetics 2017 Dec;207(4):1423-1440

Role for the IFT-A Complex in Selective Transport to the Primary Cilium.

Fu W, Wang L, Kim S, Li J, Dynlacht BD
Cell reports 2016 Nov 1;17(6):1505-1517

Chibby functions to preserve normal ciliary morphology through the regulation of intraflagellar transport in airway ciliated cells.

Siller SS, Burke MC, Li FQ, Takemaru K
Cell cycle (Georgetown, Tex.) 2015;14(19):3163-72

Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.

Raman M, Sergeev M, Garnaas M, Lydeard JR, Huttlin EL, Goessling W, Shah JV, Harper JW
Nature cell biology 2015 Oct;17(10):1356-69

Nephrocystin proteins NPHP5 and Cep290 regulate BBSome integrity, ciliary trafficking and cargo delivery.

Barbelanne M, Hossain D, Chan DP, Peränen J, Tsang WY
Human molecular genetics 2015 Apr 15;24(8):2185-200

IFT27 links the BBSome to IFT for maintenance of the ciliary signaling compartment.

Eguether T, San Agustin JT, Keady BT, Jonassen JA, Liang Y, Francis R, Tobita K, Johnson CA, Abdelhamed ZA, Lo CW, Pazour GJ
Developmental cell 2014 Nov 10;31(3):279-290

The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3.

Liew GM, Ye F, Nager AR, Murphy JP, Lee JS, Aguiar M, Breslow DK, Gygi SP, Nachury MV
Developmental cell 2014 Nov 10;31(3):265-278

Essential role of the chaperonin CCT in rod outer segment biogenesis.

Sinha S, Belcastro M, Datta P, Seo S, Sokolov M
Investigative ophthalmology & visual science 2014 May 22;55(6):3775-85

The centriolar satellite protein AZI1 interacts with BBS4 and regulates ciliary trafficking of the BBSome.

Chamling X, Seo S, Searby CC, Kim G, Slusarski DC, Sheffield VC
PLoS genetics 2014 Feb;10(2):e1004083

Structural basis for membrane targeting of the BBSome by ARL6.

Mourão A, Nager AR, Nachury MV, Lorentzen E
Nature structural & molecular biology 2014 Dec;21(12):1035-41

Visualizing renal primary cilia.

Deane JA, Verghese E, Martelotto LG, Cain JE, Galtseva A, Rosenblum ND, Watkins DN, Ricardo SD
Nephrology (Carlton, Vic.) 2013 Mar;18(3):161-8

Light-dependent phosphorylation of Bardet-Biedl syndrome 5 in photoreceptor cells modulates its interaction with arrestin1.

Smith TS, Spitzbarth B, Li J, Dugger DR, Stern-Schneider G, Sehn E, Bolch SN, McDowell JH, Tipton J, Wolfrum U, Smith WC
Cellular and molecular life sciences : CMLS 2013 Dec;70(23):4603-16

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Garcia-Gonzalo FR, Corbit KC, Sirerol-Piquer MS, Ramaswami G, Otto EA, Noriega TR, Seol AD, Robinson JF, Bennett CL, Josifova DJ, García-Verdugo JM, Katsanis N, Hildebrandt F, Reiter JF
Nature genetics 2011 Jul 3;43(8):776-84

The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia.

Jin H, White SR, Shida T, Schulz S, Aguiar M, Gygi SP, Bazan JF, Nachury MV
Cell 2010 Jun 25;141(7):1208-19

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP(BBS5 antibody) at dilution of 1:100
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP(BBS5 antibody) at dilution of 1:400
Sample type: tissue