Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- 333210 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#333210, RRID:AB_2632910
- Product name
- Alexa Fluor® 594 anti-human DcR3
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- B08-35
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human paraffin-embedded breast cancer tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with BioLegend’s Tris-Buffered Saline 20X (1.0M, pH7.4) at 95°C for 40 minutes. The tissue was washed with 0.05% Tween 20/PBS twice for five minutes, permeabilized with 0.5% Triton X-100/PBS for ten minutes, and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of anti-human DcR3 (clone B08/035) Alexa Fluor® 594 (red) and anti-Nestin (clone 10C2) Brilliant Violet 421™ (green) at 4°C overnight. The nuclei were counterstained with DRAQ5™ (blue). This image was captured with a 10X objective.
- Conjugate
- Yellow dye