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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB2135 - Provider product page
- Provider
- R&D Systems
- Product name
- Human DcR3/TNFRSF6B Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human DcR3/TNFRSF6B in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Fas, rhOPG, rhTNF R2, rhTRAIL R1, rhTRAIL R2, rhTRAIL R3, or rhTRAIL R4 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O95407- Isotype
- IgG
- Antibody clone number
- 229812
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Soluble decoy receptor 3 modulates the survival and formation of osteoclasts from multiple myeloma bone disease patients.
Colucci S, Brunetti G, Mori G, Oranger A, Centonze M, Mori C, Cantatore FP, Tamma R, Rizzi R, Liso V, Zallone A, Grano M
Leukemia 2009 Nov;23(11):2139-46
Leukemia 2009 Nov;23(11):2139-46
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- DcR3/TNFRSF6B Inhibition of Fas Ligand/TNFSF6-induced Apoptosis and Neutralization by Human DcR3/TNFRSF6B Antibody. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human Fas Ligand/TNFSF6 (20 ng/mL) activity elicited by Recombinant Human DcR3/TNFRSF6B Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB2135). The ND50 is typically 0.1-0.4 µg/mL.
