MA5-17058

antibody from Invitrogen Antibodies

Targeting: KRT5 EBS2, KRT5A

ELISA

Antibody data

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Validation data

Product number: MA5-17058 - Provider product page
Provider: Invitrogen Antibodies
Product name: Cytokeratin 5 Monoclonal Antibody (10C11E6)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-17058 targets CK5 in IHC, indirect ELISA, and WB applications and shows reactivity with Human samples. The MA5-17058 immunogen is purified recombinant fragment of human CK5 (amino acids: 316-590) expressed in E. Coli. MA5-17058 detects CK5 which has a predicted molecular weight of approximately 62.4kDa.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 10C11E6
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

KRT5 protein structure - MA5-17058 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CK5 using CK5 monoclonal antibody (Product # MA5-17058) in A431 (1) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CK5 using CK5 monoclonal antibody (Product # MA5-17058) in A431 (1) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Cytokeratin 5 was achieved by transfecting A-431 cells with Cytokeratin 5 specific siRNAs (Silencer® select Product # s7956, S7957). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Cytokeratin 5 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Cytokeratin 5 Monoclonal Antibody (10C11E6) (Product # MA5-17058, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A28177, at 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Cytokeratin 5.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Cytokeratin 5 Monoclonal Antibody (10C11E6) (Product # MA5-17058) and a 60kDa band corresponding to Cytokeratin 5 was observed across the cell lines tested except MCF7, IMR-32 and SH-SY5Y. Whole cell extracts (30 µg lysate) of MCF 10A (Lane 1), MCF7 (Lane 2), A-431 (Lane 3), IMR-32 (Lane 4) and SH-SY5Y (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).