Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- NBP1-97730AF488 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-97730AF488, RRID:AB_2725810
- Product name
- Mouse Monoclonal Cytokeratin 5/8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. RCK102 is reacting with cytokeratin 5 and cytokeratin 8. This monoclonal antibody recognizes virtually all epithelial tissues and carcinomas.
- Reactivity
- Human, Mouse, Rat, Canine, Feline, Hamster, Porcine, Rabbit
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730AF488] - An intracellular stain was performed on NTERA-2 cells with Cytokeratin 5/8 (RCK102) antibody NBP1-97730AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Cytokeratin 5/8 Antibody (RCK102) [Alexa Fluor® 488] [NBP1-97730AF488] - An intracellular stain was performed on HeLa cells with Cytokeratin 5/8 (RCK102) antibody NBP1-97730AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.