NBP1-97730AF488

antibody from Novus Biologicals

Targeting: KRT5 EBS2, KRT5A

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-97730AF488

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-97730AF488, RRID:AB_2725810

Product name

Mouse Monoclonal Cytokeratin 5/8 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified. RCK102 is reacting with cytokeratin 5 and cytokeratin 8. This monoclonal antibody recognizes virtually all epithelial tissues and carcinomas.

Reactivity

Human, Mouse, Rat, Canine, Feline, Hamster, Porcine, Rabbit

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C in the dark.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730AF488] - An intracellular stain was performed on NTERA-2 cells with Cytokeratin 5/8 (RCK102) antibody NBP1-97730AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Cytokeratin 5/8 Antibody (RCK102) [Alexa Fluor® 488] [NBP1-97730AF488] - An intracellular stain was performed on HeLa cells with Cytokeratin 5/8 (RCK102) antibody NBP1-97730AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.