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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP2-61931 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Cytokeratin 5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 5 Antibody [NBP2-61931] - Total protein from human A431 cells and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cytokeratin 5 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-61931] - Analysis of a FFPE tissue section of human skin using 1:1000 dilution of rabbit anti-Cytokeratin 5 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Cytokeratin 5 antibody generated a specific membrane cytoplasmic staining in the epithelial cells of the skin tissue. The signal was highest in the keratinocytes while dermis was largely negative for this Cytokeratin 5 (KRT5).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Cytokeratin 5 Antibody [NBP2-61931] - An intracellular stain was performed on A431 cells with Cytokeratin 5 Antibody NBP2-61931AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
