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- Western blot [3]
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- Immunohistochemistry [2]
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- Product number
- MA5-15347 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 5 Monoclonal Antibody (3E2F1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15347 targets Cytokeratin 5 in IHC and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 3E2F1
- Concentration
- Conc. Not Determined
Submitted references Correlation between salivary estrogen levels and oral epithelial cytokeratin 5 expression.
Handajani J, Effendi N, Sosroseno W
F1000Research 2020;9:186
F1000Research 2020;9:186
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cytokeratin 5 using a Cytokeratin 5 monoclonal antibody (Product # MA5-15347) against a truncated CK5 recombinant protein
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Cytokeratin 5 was achieved by transfecting A-431 with Cytokeratin 5 specific siRNAs (Silencer® select Product # s7956, S7957). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Cytokeratin 5 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Cytokeratin 5 Monoclonal Antibody (3E2F1) (Product # MA5-15347, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Cytokeratin 5.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Cytokeratin 5 Monoclonal Antibody (3E2F1)(Product # MA5-15347) and a 60kDa band corresponding to Cytokeratin 5 was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of MCF 10A (Lane 1), MCF7 (Lane 2), A-431 (Lane 3), HaCaT (Lane 4), IMR-32 (Lane 5) and SH-SY5Y (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Cytokeratin 5 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytokeratin 5 Monoclonal Antibody (3E2F1) (Product # MA5-15347) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytoskeletal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human esophagus epithelium (A), salivary gland basal cell (B), lung squamous cell carcinoma (C), endometrium admosquamous carcinoma (D), showing cytoplasmic and membrane localization using Cytokeratin 5 monoclonal antibody (Product # MA5-15347) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human skin tissues using Cytokeratin 5 monoclonal antibody (Product # MA5-15347).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Palatal epithelial cells stained by Papanicolaou (x400) ( A ) and immunohistologically stained for cytokeratin 5 ( B ) (x400). Basal cells are stained as blue color. Intermediate and superficial are stained as pink and orange color, respectively. Cytokeratin 5 protein is stained as brown color in nucleus and cytoplasm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. The correlation between the levels of salivary estrogen and cytokeratin 5-positive oral epithelial cells. *p
