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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AM06747PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM06747PU-N, RRID:AB_11218156
- Product name
- anti Cytokeratin 5
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant fragment of Human CK5 expressed in E. Coli.
- Reactivity
- Human, Mouse, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2C2
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Left: Western blot analysis using Cytokeratin 5 antibody Cat.-No AM06747PU-N against Human CK5 (AA: 158-272) recombinant protein (Expected MW is 33.3 kDa). Right: Western blot analysis using Cytokeratin 5 antibody Cat.-No AM06747PU-N against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Left: Immunofluorescence analysis of HeLa cells. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Right: Immunofluorescence analysis of HeLa cells using Cytokeratin 5 antibody Cat.-No AM06747PU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded breast cancer (left) and stomach (right) tissues using Cytokeratin 5 antibody Cat.-No AM06747PU-N with DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of HeLa cells using Cytokeratin 5 antibody Cat.-No AM06747PU-N (green) and negative control (red).
