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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- AMM81116 - Provider product page
- Provider
- EnkiLife Biotech Co., Ltd.
- Product name
- CK5 Mouse Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Affinity Purification
- Reactivity
- Human, Mouse, Rat, Rabbit, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- Monoclonal
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western blot analysis using CK5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western blot analysis using CK5 mouse mAb against PC-12(1)C2C12(2)C6(3)L1210(4)CHO3D10(5)cell lysate.
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using CK5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using CK5 mouse mAb with DAB staining.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using CK5 mouse mAb with DAB staining.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Rat kidney using CCL2 mouse mAb with DAB staining.
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using CK5 mouse mAb (green) and negative control (red).
