LS-B13005

antibody from LSBio

Targeting: AQP1 CHIP28, CO

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Enhanced validation)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B13005

Provider

LSBio

Product name

IHC-plus™ AQP1 / Aquaporin 1 Antibody LS-B13005

Antibody type

Polyclonal

Description

Caprylic acid and ammonium sulfate precipitation

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: AQP1 antibody at 16µg/ml Lane 1: Rat heart tissue Lane 2: Mouse skeletal muscle tissue Lane 3: A549 whole cell lysate Lane 4: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29, 20, 24, 17 kDa Observed band size: 29 kDa

Immunoprecipitation

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating AQP1 in Mouse skeletal muscle tissue Lane 1: Rabbit control IgG instead of AQP1 Antibody in Mouse skeletal muscle tissue.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: AQP1 Antibody (8µg) + Mouse skeletal muscle tissue (500µg) Lane 3: Mouse skeletal muscle tissue (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating AQP1 in Mouse skeletal muscle tissue Lane 1: Rabbit control IgG instead of AQP1 Antibody in Mouse skeletal muscle tissue.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: AQP1 Antibody (8µg) + Mouse skeletal muscle tissue (500µg) Lane 3: Mouse skeletal muscle tissue (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating AQP1 in Mouse skeletal muscle tissue Lane 1: Rabbit control IgG instead of AQP1 Antibody in Mouse skeletal muscle tissue.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: AQP1 Antibody (8µg) + Mouse skeletal muscle tissue (500µg) Lane 3: Mouse skeletal muscle tissue (10µg)