F54638

antibody from NSJ Bioreagents

Targeting: PCDHAC2 PCDH-ALPHA-C2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54638

Provider

NSJ Bioreagents

Product name

Protocadherin alpha-C2 Antibody / PCDHAC2

Antibody type

Polyclonal

Description

This highly specific Protocadherin alpha-C2 antibody is suitable for use in Flow cytometry/Immunofluorescence/Immunohistochemistry/Western blot applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Protocadherin alpha-C2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human NCI-H460 cell lysate with Protocadherin alpha-C2 antibody. Predicted molecular weight ~109 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of human NCI-H460 cells with Protocadherin alpha-C2 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human brain tissue with Protocadherin alpha-C2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of NCI-H460 cells with Protocadherin alpha-C2 antibody; Blue=isotype control, Green= Protocadherin alpha-C2 antibody.