Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00054474-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00054474-M01, RRID:AB_539997
- Product name
- Mouse Monoclonal Cytokeratin 20 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. KRT20 - keratin 20
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - Analysis of KRT20 expression in transfected 293T cell line by KRT20 monoclonal antibody (M01), clone 2G3-1C8.Lane 1: KRT20 transfected lysate(48.5 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - KRT20 monoclonal antibody (M01), clone 2G3-1C8 Analysis of KRT20 expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - Detection limit for recombinant GST tagged KRT20 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - Analysis of monoclonal antibody to KRT20 on U-2 OS cell. Antibody concentration 10 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - Analysis of monoclonal antibody to KRT20 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - Analysis of monoclonal antibody to KRT20 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 3 ug/ml.