MA1-35556

antibody from Invitrogen Antibodies

Targeting: KRT20 CK20, K20, MGC35423

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA1-35556

Provider

Invitrogen Antibodies

Product name

Anti-Cytokeratin 20 Monoclonal Antibody (Ks 20.8)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

For IHC applications, pretreatment with protease is recommended.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

Ks 20.8

Vial size

1 mL

Concentration

not determined

Storage

2-8°C

References

Submitted references

Detection and analysis of multiple biomarkers in ovarian cancer: clinical significance in diagnosis, treatment, and prognosis evaluation.

Ji R, Li Y, He C, Zhu X, He A, Lu Y
Gland surgery 2020 Dec;9(6):2175-2186

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Complex APC germline mutation associated metaplasia and intraepithelial neoplasia (CAM-IEN) of the gallbladder.

Böger C, Haag J, Egberts JH, Röcken C
Pathology, research and practice 2016 Jan;212(1):54-8

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Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital.

Sauter JL, Ambaye AB, Mount SL
Diagnostic cytopathology 2015 Sep;43(9):688-95

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Ectopic Atoh1 expression drives Merkel cell production in embryonic, postnatal and adult mouse epidermis.

Ostrowski SM, Wright MC, Bolock AM, Geng X, Maricich SM
Development (Cambridge, England) 2015 Jul 15;142(14):2533-44

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Adenocarcinoma of the thymus, enteric type: report of 2 cases, and proposal for a novel subtype of thymic carcinoma.

Moser B, Schiefer AI, Janik S, Marx A, Prosch H, Pohl W, Neudert B, Scharrer A, Klepetko W, Müllauer L
The American journal of surgical pathology 2015 Apr;39(4):541-8

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Miliary brain metastases from occult lung adenocarcinoma: Radiologic and histopathologic confirmation.

Kahveci R, Gürer B, Kaygusuz G, Sekerci Z
Journal of neurosciences in rural practice 2012 Sep;3(3):386-9

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Primary mucinous cystadenoma of the spermatic cord within the inguinal canal.

Kim JY, Lee YT, Kang HJ, Lee CH
Diagnostic pathology 2012 Oct 8;7:139

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Adenocarcinoma with sarcomatous dedifferentiation arising from mature cystic teratoma of the anterior mediastinum.

Kim JY, Lee CH, Park WY, Kim JY, Kim AR, Shin N, Park DY, Huh GY
Pathology, research and practice 2012 Dec 15;208(12):741-5

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Ovarian fibromas with heavy deposition of hyaline globules: a diagnostic pitfall.

Michal M, Kacerovska D, Mukensnabl P, Petersson F, Danis D, Adamkov M, Kazakov DV
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2009 Jul;28(4):356-61

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Renal angiomyoadenomatous tumor: morphologic, immunohistochemical, and molecular genetic study of a distinct entity.

Michal M, Hes O, Nemcova J, Sima R, Kuroda N, Bulimbasic S, Franco M, Sakaida N, Danis D, Kazakov DV, Ohe C, Hora M
Virchows Archiv : an international journal of pathology 2009 Jan;454(1):89-99

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of KRT20 was achieved by transfecting HT 29 cells with KRT20 specific siRNAs (Silencer® select Product # s29040, Product # s29041). Western blot analysis (Fig. a) was performed using whole cell extracts from the KRT20 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed using KRT20 Monoclonal Antibody (Product # MA1-35556) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to KRT20.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Cytokeratin 20 Monoclonal Antibody (Product # MA1-35556) on whole cell extracts (30 µg lysate) of HT-29 (Lane 1), HCT 116 (Lane 2) and MCF7 (Lane 3) and 48.48 kDa band corresponding to Cytokeratin 20 was observed only in HT-29 which is reported to be the positive model. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:50 dilution) and detected by Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).