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- Immunocytochemistry [3]
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- Product number
- PA5-22125 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 20 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa. Predicted reactivity: Mouse (82%), Rat (83%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.88 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The mitochondrial deoxyguanosine kinase is required for cancer cell stemness in lung adenocarcinoma.
Lin S, Huang C, Sun J, Bollt O, Wang X, Martine E, Kang J, Taylor MD, Fang B, Singh PK, Koomen J, Hao J, Yang S
EMBO molecular medicine 2019 Dec;11(12):e10849
EMBO molecular medicine 2019 Dec;11(12):e10849
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cytokeratin 20 in paraformaldehyde-fixed HeLa cells using a Cytokeratin 20 polyclonal antibody (Product # PA5-22125) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20 (Product # PA5-22125) antibody at 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20 (Product # PA5-22125) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cytokeratin 20 Polyclonal Antibody detects Cytokeratin 20 protein at cytoplasm on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. Cytokeratin 20 Polyclonal Antibody (Product # PA5-22125) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded HT29 xenograft, using Cytokeratin 20 (Product # PA5-22125) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 DGUOK is required for cancer cell stemness in lung adenocarcinoma cells A, B Flow cytometric assay using ALDEFLUOR (A) and anti-CD166 antibody (B) showing the effects of DGUOK depletion on the proportion of ALDH + cells (A) and CD166 + cells (B) in lung adenocarcinoma cell lines. C, D Representative images and quantitation of the effects of DGUOK depletion on lung tumor sphere formation by lung adenocarcinoma cells. E Cell survival assay using annexin V/PI staining and flow cytometric to determine the effects of DGUOK depletion on H1650 cell survival under adherent and non-adherent (30 h in lung sphere formation conditions) conditions. F Quantitation of apoptotic cells in (E) from three independent biological replicates. G, H Representative immunofluorescence staining showing the effects of DGUOK KO on expression of CK20 in Matrigel-embedded H1650 (G) and A549 (H) tumor spheres. FBS was added at day 5 to induce CD20 expression. Data information: Data are shown as mean +- SD. n = 3 in (D and F). P values were determined by two-tailed, two-sample Student's t -test. Experiments were repeated with three biological replicates with similar results.
