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antibody from Invitrogen Antibodies
Targeting: APOBEC3C
APOBEC1L, ARDC2, ARDC4, ARP5, bK150C2.3, PBI
Western blot
Immunoprecipitation
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- Antibody Data
- Antigen structure
- References [1]
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- Product number
- PA5-27629 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- APOBEC3C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NCI-H929. Predicted reactivity: Rhesus Monkey (84%), Chimpanzee (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A Single Nucleotide Polymorphism in Human APOBEC3C Enhances Restriction of Lentiviruses.
Wittkopp CJ, Adolph MB, Wu LI, Chelico L, Emerman M
PLoS pathogens 2016 Oct;12(10):e1005865
PLoS pathogens 2016 Oct;12(10):e1005865
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 5 Purified APOBEC3C I188 forms dimers in solution. (A) Size exclusion chromatography profiles of the APOBEC3C S188 and I188 from a 10mL G200 Superdex column were used to calculate the oligomerization states of the enzymes. Molecular weights were calculated by comparing to a calibration curve (see inset on right). When APOBEC3C S188 and APOBEC3C I188 were loaded onto the column, APOBEC3C S188 was a monomer in solution (apparent MW 21 kD in peak fraction), whereas APOBEC3C I188 could form dimers (apparent MW 42 kD in peak fraction) in addition to monomers (apparent MW 21 kD in peak fraction). Chromatograms were made using the integrated gel band intensities from three independent experiments of each protein fraction after resolution by SDS-PAGE. A representative Western blot of the size exclusion chromatography fractions is shown. (B) A3C S188 or I188 were incubated in the absence or presence of 10 muM bis(sulfosuccinimidyl)suberate (BS3) crosslinker (indicated as -BS3 or +BS3) and then visualized through SDS-PAGE and Western blotting. The Western blot demonstrates that A3C S188 remained monomeric in the presence of crosslinker, whereas A3C I188 was both monomeric and dimeric in the presence of the crosslinker. Molecular weight standards are indicated.
