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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Product number
- PA5-80345 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- APOBEC3B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human APOBEC3B.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- APOBEC3B Immunoprecipitation using: Lane A: 0.5 mg 293T Whole Cell Lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate 4 µL with APOBEC3B Polyclonal Antibody (Product # PA5-80345) and 15 µL of 50 % Protein G agarose. Primary antibody: APOBEC3B Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 46 kDa.
