16520-1-AP

antibody from Proteintech Group

Targeting: DES CMD1I, CSM1, CSM2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

16520-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16520-1-AP, RRID:AB_2292918

Product name

Desmin antibody

Antibody type

Polyclonal

Description

Desmin antibody (Cat. #16520-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

RASSF4 Attenuates Metabolic Dysfunction-Associated Steatotic Liver Disease Progression via Hippo Signaling and Suppresses Hepatocarcinogenesis.

Xu C, Fang T, Qu J, Miao Y, Tian L, Zhang M, Zhuang H, Sun B, Chen L
Cellular and molecular gastroenterology and hepatology 2024;18(2):101348

---

Crocin Inhibits Orbital Fibroblasts Fibrosis in Thyroid-Associated Ophthalmopathy.

Xu S, Mao H
Current eye research 2024 Mar;49(3):330-337

---

RRM2 promotes the proliferation of chicken myoblasts, inhibits their differentiation and muscle regeneration.

Chen B, Zhang Y, Niu Y, Wang Y, Liu Y, Ji H, Han R, Tian Y, Liu X, Kang X, Li Z
Poultry science 2024 Mar;103(3):103407

---

LncRNA-SNHG5 mediates activation of hepatic stellate cells by regulating NF2 and Hippo pathway.

Zhang R, Zhan Y, Lang Z, Li Y, Zhang W, Zheng J
Communications biology 2024 Mar 4;7(1):266

---

FTO-mediated m6A modification of serum amyloid A2 mRNA promotes podocyte injury and inflammation by activating the NF-κB signaling pathway.

Lang Y, Wang Q, Sheng Q, Lu S, Yang M, Kong Z, Gao Y, Fan X, Shen N, Wang R, Lv Z
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Jan 31;38(2):e23409

---

The CXCR4-AT1 axis plays a vital role in glomerular injury via mediating the crosstalk between podocyte and mesangial cell.

Wu Q, Zhou S, Xu D, Meng P, Chen Q, Wang X, Li X, Chen S, Ye H, Ye W, Xiong Y, Li J, Miao J, Shen W, Lin X, Hou FF, Liu Y, Zhang Y, Zhou L
Translational research : the journal of laboratory and clinical medicine 2024 Feb;264:15-32

---

Interleukin-6 trans-signaling regulates monocyte chemoattractant protein-1 production in immune-mediated necrotizing myopathy.

Ma X, Gao HJ, Ge HZ, Zhang Q, Bu BT
Rheumatology (Oxford, England) 2024 Feb 23;

---

Targeted inhibition of autophagy in hepatic stellate cells by hydroxychloroquine: An effective therapeutic approach for the treatment of liver fibrosis.

Hou LS, Zhai XP, Zhang YW, Xing JH, Li C, Zhou SY, Zhu XH, Zhang BL
Liver international : official journal of the International Association for the Study of the Liver 2024 Aug;44(8):1937-1951

---

Activating SIRT1 deacetylates NF-κB p65 to alleviate liver inflammation and fibrosis via inhibiting NLRP3 pathway in macrophages.

He S, Wang Y, Liu J, Li P, Luo X, Zhang B
International journal of medical sciences 2023;20(4):505-519

---

Upregulated desmin/integrin β1/MAPK axis promotes elastic cartilage regeneration with increased ECM mechanical strength.

Zhang W, Lu W, Yu Q, Liu X, Jiang H
International journal of biological sciences 2023;19(9):2740-2755

---

Combining Porous Se@SiO(2) Nanocomposites and dECM Enhances the Myogenic Differentiation of Adipose-Derived Stem Cells.

Zhang YC, Yang YX, Liu Y, Liu XJ, Dai JH, Gao RS, Hu YY, Fei WY
International journal of nanomedicine 2023;18:7661-7676

---

Nano-calcipotriol as a potent anti-hepatic fibrosis agent.

Zhang Y, Wang L, Shao J, Liu Y, Lu Y, Yang J, Xu S, Zhang J, Li M, Liu X, Zheng M
MedComm 2023 Oct;4(5):e354

---

Diet-induced obesity augments ischemic myopathy and functional decline in a murine model of peripheral artery disease.

Fletcher E, Miserlis D, Sorokolet K, Wilburn D, Bradley C, Papoutsi E, Wilkinson T, Ring A, Ferrer L, Haynatzki G, Smith RS, Bohannon WT, Koutakis P
Translational research : the journal of laboratory and clinical medicine 2023 Oct;260:17-31

---

CXCL5 promotes lipotoxicity of hepatocytes through upregulating NLRP3/Caspase-1/IL-1β signaling in Kupffer cells and exacerbates nonalcoholic steatohepatitis in mice.

Qi J, Yan X, Li L, Qiu K, Huang W, Zhou Z
International immunopharmacology 2023 Oct;123:110752

---

Peptide-dendrimer-reinforced bioinks for 3D bioprinting of heterogeneous and biomimetic in vitro models.

Zhou K, Ding R, Tao X, Cui Y, Yang J, Mao H, Gu Z
Acta biomaterialia 2023 Oct 1;169:243-255

---

Therapeutic Myogenesis Induced by Ultrasound Exposure in a Volumetric Skeletal Muscle Loss Injury Model.

Mohamad Yusoff F, Nakashima A, Kajikawa M, Kishimoto S, Maruhashi T, Higashi Y
The American journal of sports medicine 2023 Nov;51(13):3554-3566

---

Sweroside attenuates podocyte injury and proteinuria in part by activating Akt/BAD signaling in mice.

Huang M, Yang Y, Chen Y, Li Y, Qin S, Xiao L, Long X, Hu K, Li Y, Ying H, Ding Y
Journal of cellular biochemistry 2023 Nov;124(11):1749-1763

---

Resveratrol protects renal damages induced by periodontitis via preventing mitochondrial dysfunction in rats.

Li X, Liu XC, Ding X, Liu XM, Cao NB, Deng Y, Hou YB, Yu WX
Oral diseases 2023 May;29(4):1812-1825

---

Rhein Inhibited Ferroptosis and EMT to Attenuate Diabetic Nephropathy by Regulating the Rac1/NOX1/β-Catenin Axis.

Xiong D, Hu W, Han X, Cai Y
Frontiers in bioscience (Landmark edition) 2023 May 25;28(5):100

---

The super-healing MRL strain promotes muscle growth in muscular dystrophy through a regenerative extracellular matrix.

O'Brien JG, Willis AB, Long AM, Kwon J, Lee G, Li F, Page PGT, Vo AH, Hadhazy M, Crosbie RH, Demonbreun AR, McNally EM
bioRxiv : the preprint server for biology 2023 Jun 30;

---

N-acetylglucosamine-bearing polymers mimicking O-GlcNAc-modified proteins elicit anti-fibrotic activities in myofibroblasts and activated stellate cells.

Ise H, Araki Y, Song I, Akatsuka G
Glycobiology 2023 Jan 8;33(1):17-37

---

Single-cell transcriptome analysis reveals liver injury induced by glyphosate in mice.

Wu J, Sun X, Wu C, Hong X, Xie L, Shi Z, Zhao L, Du Q, Xiao W, Sun J, Wang J
Cellular & molecular biology letters 2023 Feb 4;28(1):11

---

Host Desmin Interacts with RABV Matrix Protein and Facilitates Virus Propagation.

Zhang W, Liu Y, Li M, Zhu J, Li X, Luo TR, Liang J
Viruses 2023 Feb 4;15(2)

---

Synergistic Effect of Hydrogen and 5-Aza on Myogenic Differentiation through the p38 MAPK Signaling Pathway in Adipose-Derived Mesenchymal Stem Cells.

Fei W, Pang E, Hou L, Dai J, Liu M, Wang X, Xie B, Wang J
International journal of stem cells 2023 Feb 28;16(1):78-92

---

Single-cell transcriptomic dissection of the cellular and molecular events underlying the triclosan-induced liver fibrosis in mice.

Bai YM, Yang F, Luo P, Xie LL, Chen JH, Guan YD, Zhou HC, Xu TF, Hao HW, Chen B, Zhao JH, Liang CL, Dai LY, Geng QS, Wang JG
Military Medical Research 2023 Feb 22;10(1):7

---

Qian Yang Yu Yin granule improves hypertensive renal damage: A potential role for TRPC6-CaMKKβ-AMPK-mTOR-mediated autophagy.

Ma S, Xu J, Zheng Y, Li Y, Wang Y, Li H, Fang Z, Li J
Journal of ethnopharmacology 2023 Feb 10;302(Pt A):115878

---

Inhibition of the lncRNA 585189 prevents podocyte injury and mitochondria dysfunction by promoting hnRNP A1 and SIRT1 in diabetic nephropathy.

Chen H, Liu Y, Zhang T, Huang T, Lang Y, Sheng Q, Liu Y, Kong Z, Gao Y, Lu S, Yang M, Li X, Wang R, Lv Z
Molecular and cellular endocrinology 2023 Dec 1;578:112065

---

Upregulated estrogen receptors impairs myogenesis and elevates adipogenesis related factor levels in the paravertebral muscles of patients with idiopathic scoliosis.

Shu Q, Yang G, Tang M, Guo C, Zhang H, Li J
Biochemical and biophysical research communications 2023 Apr 16;652:22-30

---

GL-V9 ameliorates liver fibrosis by inhibiting TGF-β/smad pathway.

Guo Y, Tian G, Chen X, Hou Y, Zhang X, Xue X, Zhao L, Wu Y
Experimental cell research 2023 Apr 1;425(1):113521

---

Selective Inhibition of Histone Deacetylase Class IIa With MC1568 Ameliorates Podocyte Injury.

He X, Sun T, Zhang P, Xia Z, Gao C, Ren H, Ji D
Frontiers in medicine 2022;9:848938

---

Long Noncoding RNA ENSG00000254693 Promotes Diabetic Kidney Disease via Interacting with HuR.

Yu Q, Lin J, Ma Q, Li Y, Wang Q, Chen H, Liu Y, Liu B
Journal of diabetes research 2022;2022:8679548

---

Tanreqing Injection Regulates Cell Function of Hypoxia-Induced Human Pulmonary Artery Smooth Muscle Cells (HPASMCs) through TRPC1/CX3CL1 Signaling Pathway.

Xiang Y, Zheng F, Zhang Q, Zhang R, Pan H, Pang Z, Dai S, Zhang Y, Wu Y, Yao L, Su M, Lan L
Oxidative medicine and cellular longevity 2022;2022:3235102

---

Expression patterns and correlation analyses of muscle-specific genes in the process of sheep myoblast differentiation.

Wang H, Dou M, Li J, Cao P, Li J, Guo T, Zhao D, Khan A, Li Y, Li B, Qin J, Du R
In vitro cellular & developmental biology. Animal 2022 Oct;58(9):798-809

---

Antagonistic control of active surface integrins by myotubularin and phosphatidylinositol 3-kinase C2β in a myotubular myopathy model.

Samsó P, Koch PA, Posor Y, Lo WT, Belabed H, Nazare M, Laporte J, Haucke V
Proceedings of the National Academy of Sciences of the United States of America 2022 Oct 4;119(40):e2202236119

---

Targeting A-kinase anchoring protein 12 phosphorylation in hepatic stellate cells regulates liver injury and fibrosis in mouse models.

Ramani K, Mavila N, Abeynayake A, Tomasi ML, Wang J, Matsuda M, Seki E
eLife 2022 Oct 4;11

---

Targeted inhibition of the immunoproteasome blocks endothelial MHC class II antigen presentation to CD4(+) T cells in chronic liver injury.

Zhang Y, Yang X, Bi T, Wu X, Wang L, Ren Y, Ou Y, Xie C, Li K, Ran H, Wang J, Zhao F, Shui P, Qing J
International immunopharmacology 2022 Jun;107:108639

---

Sestrin2 remedies podocyte injury via orchestrating TSP-1/TGF-β1/Smad3 axis in diabetic kidney disease.

Song S, Shi C, Bian Y, Yang Z, Mu L, Wu H, Duan H, Shi Y
Cell death & disease 2022 Jul 30;13(7):663

---

Bone marrow mesenchymal stem cells inhibit hepatic fibrosis via the AABR07028795.2/rno-miR-667-5p axis.

Feng Y, Li Y, Xu M, Meng H, Dai C, Yao Z, Lin N
Stem cell research & therapy 2022 Jul 28;13(1):375

---

MYPT1 reduction is a pathogenic factor of erectile dysfunction.

Zhao W, Sun J, Yao LY, Hang D, Li YQ, Chen CP, Zhou YW, Chen X, Tao T, Wei LS, Zheng YY, Ge X, Li CJ, Xin ZC, Pan Y, Wang XZ, He WQ, Zhang XN, Yao B, Zhu MS
Communications biology 2022 Jul 25;5(1):744

---

Construction and identification of an immortalized rat intestinal smooth muscle cell line.

Chen H, Li X, Gao L, Zhang D, Han T
Neurogastroenterology and motility 2022 Aug;34(8):e14359

---

ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.

Yu S, Zhang R, Shen Q, Zhu Z, Zhang J, Wu X, Zhao W, Li N, Yang F, Wei H, Hua J
Frontiers in cell and developmental biology 2021;9:712224

---

The important roles and molecular mechanisms of annexin A(2) autoantibody in children with nephrotic syndrome.

Ye Q, Zhang Y, Zhuang J, Bi Y, Xu H, Shen Q, Liu J, Fu H, Wang J, Feng C, Tang X, Liu F, Gu W, Zhao F, Zhang J, Qin Y, Shang S, Shen H, Chen X, Shen H, Liu A, Xia Y, Lu Z, Shu Q, Mao J
Annals of translational medicine 2021 Sep;9(18):1452

---

Vitellogenin 2 promotes muscle development and stimulates the browning of white fat.

Li Y, Sun X, Bai Y, Ji Y, Ren H, Yu X, Yan Y, He X, Dong Y, Zhang L, Luo X, Wang H
Aging 2021 Oct 5;13(19):22985-23003

---

Podocyte apoptosis in diabetic nephropathy by BASP1 activation of the p53 pathway via WT1.

Zhang Y, Xu C, Ye Q, Tong L, Jiang H, Zhu X, Huang L, Lin W, Fu H, Wang J, Persson PB, Lai EY, Mao J
Acta physiologica (Oxford, England) 2021 May;232(1):e13634

---

Dual Regeneration of Muscle and Nerve by Intramuscular Infusion of Mitochondria in a Nerve Crush Injury Model.

Sheu ML, Shen CC, Tsou HK, Yang MY, Su HL, Sheehan J, Chang MH, Chen HS, Pan HC
Neurosurgery 2021 Jun 15;89(1):E49-E59

---

Clinical trajectory of a patient with filaminopathy who developed arrhythmogenic cardiomyopathy, myofibrillar myopathy, and multiorgan tumors.

Matsumura T, Inoue K, Toyooka K, Inoue M, Iida A, Saito Y, Nishikawa T, Moriuchi K, Beck G, Nishino I, Fujimura H
Neuromuscular disorders : NMD 2021 Dec;31(12):1282-1286

---

BMP9 attenuates occurrence of venous malformation by maintaining endothelial quiescence and strengthening vessel walls via SMAD1/5/ID1/α-SMA pathway.

Li Y, Shang Q, Li P, Yang Z, Yang J, Shi J, Ge S, Wang Y, Fan X, Jia R
Journal of molecular and cellular cardiology 2020 Oct;147:92-107

---

Neointimal hyperplasia in the inferior vena cava of adenine-induced chronic kidney disease rats with aortocaval fistulas.

Du J, Liang L, Liu S, Yang X, Cao S, Zhang H, Su H, Gong X, Chen Q, Kong X, Xu D
Clinical and experimental nephrology 2020 Nov;24(11):1007-1014

---

TRAF6 Activates Fibroblasts to Cancer-Associated Fibroblasts through FGF19 in Tumor Microenvironment to Benefit the Malignant Phenotype of Melanoma Cells.

Guo Y, Zhang X, Zeng W, Zhang J, Cai L, Wu Z, Su J, Xiao Y, Liu N, Tang L, Xu X, Chen X, Peng C
The Journal of investigative dermatology 2020 Nov;140(11):2268-2279.e11

---

Multimeric conformation of type III intermediate filaments but not the filamentous conformation exhibits high affinity to lipid bilayers.

Hwang B, Ise H
Genes to cells : devoted to molecular & cellular mechanisms 2020 Jun;25(6):413-426

---

Effect of sarcolipin-mediated cell transdifferentiation in sarcopenia-associated skeletal muscle fibrosis.

Chen QN, Fan Z, Lyu AK, Wu J, Guo A, Yang YF, Chen JL, Xiao Q
Experimental cell research 2020 Apr 1;389(1):111890

---

miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepatic stellate cells.

Ju B, Nie Y, Yang X, Wang X, Li F, Wang M, Wang C, Zhang H
Journal of cellular and molecular medicine 2019 Jun;23(6):3824-3832

---

Molecular network of miR-1343 regulates the pluripotency of porcine pluripotent stem cells via repressing OTX2 expression.

Xie Y, Cao H, Zhang Z, Zhang S, Wang H
RNA biology 2019 Jan;16(1):82-92

---

Yes-associated protein regulates podocyte cell cycle re-entry and dedifferentiation in adriamycin-induced nephropathy.

Xie K, Xu C, Zhang M, Wang M, Min L, Qian C, Wang Q, Ni Z, Mou S, Dai H, Pang H, Gu L
Cell death & disease 2019 Dec 4;10(12):915

---

Protein phosphatase 2A modulates podocyte maturation and glomerular functional integrity in mice.

Zhu X, Ye Y, Xu C, Gao C, Zhang Y, Zhou J, Lin W, Mao J
Cell communication and signaling : CCS 2019 Aug 6;17(1):91

---

Zhen-wu-tang protects against podocyte injury in rats with IgA nephropathy via PPARγ/NF-κB pathway.

Liu B, He Y, Lu R, Zhou J, Bai L, Zhang P, Ye S, Wu J, Liang C, Zhou Y, Zhou J
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 May;101:635-647

---

Interleukin-17A participates in podocyte injury by inducing IL-1β secretion through ROS-NLRP3 inflammasome-caspase-1 pathway.

Yan J, Li Y, Yang H, Zhang L, Yang B, Wang M, Li Q
Scandinavian journal of immunology 2018 Apr;87(4):e12645

---

miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.

Shi J, Bei Y, Kong X, Liu X, Lei Z, Xu T, Wang H, Xuan Q, Chen P, Xu J, Che L, Liu H, Zhong J, Sluijter JP, Li X, Rosenzweig A, Xiao J
Theranostics 2017;7(3):664-676

---

Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.

Liang D, Wang Z, Yan Z, Hou S, Xu W, Wang L, Shang M, Qiao Z
Scientific reports 2017 Mar 2;7:43798

---

Prevention of Axonal Degeneration by Perineurium Injection of Mitochondria in a Sciatic Nerve Crush Injury Model.

Kuo CC, Su HL, Chang TL, Chiang CY, Sheu ML, Cheng FC, Chen CJ, Sheehan J, Pan HC
Neurosurgery 2017 Mar 1;80(3):475-488

---

Improved neurological outcome by intramuscular injection of human amniotic fluid derived stem cells in a muscle denervation model.

Chen CJ, Cheng FC, Su HL, Sheu ML, Lu ZH, Chiang CY, Yang DY, Sheehan J, Pan HC
PloS one 2015;10(5):e0124624

---

MiR-146a-5p suppresses activation and proliferation of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis through directly targeting Wnt1 and Wnt5a.

Du J, Niu X, Wang Y, Kong L, Wang R, Zhang Y, Zhao S, Nan Y
Scientific reports 2015 Nov 5;5:16163

---

MAD2B contributes to podocyte injury of diabetic nephropathy via inducing cyclin B1 and Skp2 accumulation.

Su H, Wan Q, Tian XJ, He FF, Gao P, Tang H, Ye C, Fan D, Chen S, Wang YM, Meng XF, Zhang C
American journal of physiology. Renal physiology 2015 Apr 1;308(7):F728-36

---

Effects of asiaticoside on levels of podocyte cytoskeletal proteins and renal slit diaphragm proteins in adriamycin-induced rat nephropathy.

Wang Z, Liu J, Sun W
Life sciences 2013 Sep 3;93(8):352-8

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse heart tissue were subjected to SDS PAGE followed by western blot with 16520-1-AP(DES antibody) at dilution of 1:4000

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using DES antibody 16520-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human heart using 16520-1-AP(DES antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human heart using 16520-1-AP(DES antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue