MA1-22150

antibody from Invitrogen Antibodies

Targeting: DES CMD1I, CSM1, CSM2

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-22150

Provider

Invitrogen Antibodies

Product name

Desmin Monoclonal Antibody (DE-U-10)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

Recommended positive controls: chicken gizzard.

Reactivity

Human, Mouse, Rat, Bovine, Feline, Goat, Hamster, Porcine, Rabbit

Host

Mouse

Isotype

IgG

Antibody clone number

DE-U-10

Vial size

100 µL

Concentration

9.9 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Elevated TGF β2 serum levels in Emery-Dreifuss Muscular Dystrophy: Implications for myocyte and tenocyte differentiation and fibrogenic processes.

Bernasconi P, Carboni N, Ricci G, Siciliano G, Politano L, Maggi L, Mongini T, Vercelli L, Rodolico C, Biagini E, Boriani G, Ruggiero L, Santoro L, Schena E, Prencipe S, Evangelisti C, Pegoraro E, Morandi L, Columbaro M, Lanzuolo C, Sabatelli P, Cavalcante P, Cappelletti C, Bonne G, Muchir A, Lattanzi G
Nucleus (Austin, Tex.) 2018 Jan 1;9(1):292-304

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BPAG1 isoform-b: complex distribution pattern in striated and heart muscle and association with plectin and alpha-actinin.

Steiner-Champliaud MF, Schneider Y, Favre B, Paulhe F, Praetzel-Wunder S, Faulkner G, Konieczny P, Raith M, Wiche G, Adebola A, Liem RK, Langbein L, Sonnenberg A, Fontao L, Borradori L
Experimental cell research 2010 Feb 1;316(3):297-313

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. EDMD2 fibroblast medium induces TGF beta2-dependent fibrogenic conversion in NHM cultures. (A) TGF beta2 secretion in control and EDMD2 fibroblast culture medium. (B) Quantification of myofibroblasts in cultures of control human myoblasts maintained in medium conditioned by control (control) or EDMD2 (EDMD2) fibroblasts or treated with TGF beta2 (control + TGF beta2). The number of myofibroblasts was determined by counting alpha-SMA positive mononucleated cells (200 cells/sample were counted in three independent experiments). (C) Immunofluorescence staining of collagen I (green) and desmin (red) in cycling and differentiated NHM cultured in presence of medium conditioned by control or EDMD2 fibroblasts, treated (anti-TGFbeta2) or not (NT) with anti-TGF beta2 neutralizing antibodies. Nuclei were stained with DAPI. Bar: 10um. (D) Immunofluorescence staining of ED-fibronectin (red) in cryosections of muscle tissue isolated from healthy donors (control), EDMD2 patients (EDMD2) or Becker muscular dystrophy patients (BMD). Nuclei were stained with DAPI. BMD tissue was used as positive control. In control muscle, ED-fibronectin is restricted to the area surrounding blood vessels. Bar: 10um. (E) Quantitative analysis of proliferating cells in NHM cultures maintained in medium conditioned by control or EDMD2 fibroblasts, treated (anti-TGF beta2) or not (NT) with anti-TGF beta2 neutralizing antibodies. The number of proliferating cells was determined by flow cytometry. Means