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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Flow cytometry [1]
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- Product number
- GTX61113 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX61113, RRID:AB_10620328
- Product name
- Desmin antibody [Y66]
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide corresponding to C terminus of human Desmin
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100µl
- Concentration
- 0,017 mg/ml
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Submitted references Differential drug class-specific metastatic effects following treatment with a panel of angiogenesis inhibitors.
Chung AS, Kowanetz M, Wu X, Zhuang G, Ngu H, Finkle D, Komuves L, Peale F, Ferrara N
The Journal of pathology 2012 Aug;227(4):404-16
The Journal of pathology 2012 Aug;227(4):404-16
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- A. Western blot analysis on mouse heart tissue using anti-Desmin RabMAb (catalog #GTX61113).
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of mouse heart tissue using Desmin antibody [Y66] at a dilution of 1:500 in the absence and presence of blocking peptide.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of (1) mouse heart and (2) rat heart lysates (20 ?g per lane) using Desmin antibody [Y66] at a dilution of 1:500000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of (1) Guinea pig heart and (2) Guinea pig muscle lysates (10 ?g per lane) using Desmin antibody [Y66] at a dilution of 1:100000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of (1) human skeletal muscle, (2) human fetal heart and (3) human fetal muscle lysates (20 ?g per lane) using Desmin antibody [Y66] at a dilution of 1:500000.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FACS analysis of permeabilized HeLa cells using Desmin antibody [Y66] at a dilution of 1:20 (red) or a control IgG (green).
- Validation comment
- FACS
