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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [2]
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- Product number
- LS-C815472 - Provider product page
- Provider
- LSBio
- Product name
- DES / Desmin Antibody (Preservative Free) LS-C815472
- Antibody type
- Polyclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-DES rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse muscle tissue lysate. Lane B: Mouse heart tissue lysate. Lysates/proteins at 30 ug per laneSecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 55 kDa.
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- DES was immunoprecipitated using: Lane A: 0.5 mg Mouse heart tissue ysate Whole Cell Lysate. Lane B: 0.5 mg A431 Whole Cell Lysate. 4 uL anti-DES rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-DES rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 55 kDa.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- DES was immunoprecipitated using: Lane A: 0.5 mg Mouse heart tissue ysate Whole Cell Lysate. Lane B: 0.5 mg A431 Whole Cell Lysate. 4 uL anti-DES rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-DES rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 55 kDa.
