Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- M01948-3 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Desmin Antibody Picoband™ (monoclonal, 2B5)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for Desmin detection. Tested with WB, IHC-P, FCM in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2B5
- Vial size
- 100μg/vial
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Submitted references MicroRNA-223 downregulation promotes HBx-induced podocyte pyroptosis by targeting the NLRP3 inflammasome.
Satellite cells isolated from skeletal muscle will proliferate faster in WENS yellow feather chicks.
Intranasal administration of human umbilical cord mesenchymal stem cells-conditioned medium enhances vascular remodeling after stroke.
Low dose of IGF-I increases cell size of skeletal muscle satellite cells via Akt/S6K signaling pathway.
Identification of apoptosis-related microRNAs and their target genes in myocardial infarction post-transplantation with skeletal myoblasts.
Inhibition of pancreatic stellate cell activity by adipose-derived stem cells.
Transforming growth factor β1 induces the expression of collagen type I by DNA methylation in cardiac fibroblasts.
Neurotransmitter noradrenaline downregulate cytoskeletal protein expression of VSMCs.
Altered gene and protein expression of glucose transporter1 underlies dexamethasone inhibition of insulin-stimulated glucose uptake in chicken muscles.
Protective effect of sulodexide on podocyte injury in adriamycin nephropathy rats.
17beta-estradiol affects proliferation and apoptosis of rat prostatic smooth muscle cells by modulating cell cycle transition and related proteins.
Yu Y, Dong H, Zhang Y, Sun J, Li B, Chen Y, Feng M, Yang X, Gao S, Jiang W
Archives of virology 2022 Sep;167(9):1841-1854
Archives of virology 2022 Sep;167(9):1841-1854
Satellite cells isolated from skeletal muscle will proliferate faster in WENS yellow feather chicks.
Gao CQ, Zhang HJ, Yan HC, Yuan L, Dahanayaka S, Li HC, Wang XQ
Animal science journal = Nihon chikusan Gakkaiho 2016 Jan;87(1):126-33
Animal science journal = Nihon chikusan Gakkaiho 2016 Jan;87(1):126-33
Intranasal administration of human umbilical cord mesenchymal stem cells-conditioned medium enhances vascular remodeling after stroke.
Zhao Q, Hu J, Xiang J, Gu Y, Jin P, Hua F, Zhang Z, Liu Y, Zan K, Zhang Z, Zu J, Yang X, Shi H, Zhu J, Xu Y, Cui G, Ye X
Brain research 2015 Oct 22;1624:489-496
Brain research 2015 Oct 22;1624:489-496
Low dose of IGF-I increases cell size of skeletal muscle satellite cells via Akt/S6K signaling pathway.
Gao CQ, Zhi R, Yang Z, Li HC, Yan HC, Wang XQ
Journal of cellular biochemistry 2015 Nov;116(11):2637-48
Journal of cellular biochemistry 2015 Nov;116(11):2637-48
Identification of apoptosis-related microRNAs and their target genes in myocardial infarction post-transplantation with skeletal myoblasts.
Liu Q, Du GQ, Zhu ZT, Zhang C, Sun XW, Liu JJ, Li X, Wang YS, Du WJ
Journal of translational medicine 2015 Aug 19;13:270
Journal of translational medicine 2015 Aug 19;13:270
Inhibition of pancreatic stellate cell activity by adipose-derived stem cells.
Yu FX, Su LF, Dai CL, Wang Y, Teng YY, Fu JH, Zhang QY, Tang YH
Hepatobiliary & pancreatic diseases international : HBPD INT 2015 Apr;14(2):215-21
Hepatobiliary & pancreatic diseases international : HBPD INT 2015 Apr;14(2):215-21
Transforming growth factor β1 induces the expression of collagen type I by DNA methylation in cardiac fibroblasts.
Pan X, Chen Z, Huang R, Yao Y, Ma G
PloS one 2013;8(4):e60335
PloS one 2013;8(4):e60335
Neurotransmitter noradrenaline downregulate cytoskeletal protein expression of VSMCs.
Liu J, Liu Y, Xu Y, Wang M, Zhu Q, Li W, Wang X, Liu J, Ma B, Wu K
Experimental and molecular pathology 2013 Feb;94(1):79-83
Experimental and molecular pathology 2013 Feb;94(1):79-83
Altered gene and protein expression of glucose transporter1 underlies dexamethasone inhibition of insulin-stimulated glucose uptake in chicken muscles.
Zhao JP, Bao J, Wang XJ, Jiao HC, Song ZG, Lin H
Journal of animal science 2012 Dec;90(12):4337-45
Journal of animal science 2012 Dec;90(12):4337-45
Protective effect of sulodexide on podocyte injury in adriamycin nephropathy rats.
Chen S, Fang Z, Zhu Z, Deng A, Liu J, Zhang C
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2009 Dec;29(6):715-9
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2009 Dec;29(6):715-9
17beta-estradiol affects proliferation and apoptosis of rat prostatic smooth muscle cells by modulating cell cycle transition and related proteins.
Luo Y, Waladali W, Li S, Zheng X, Hu L, Zheng H, Hu W, Chen C
Cell biology international 2008 Aug;32(8):899-905
Cell biology international 2008 Aug;32(8):899-905
No comments: Submit comment
Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Desmin using anti-Desmin antibody (M01948-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates Lane 2: rat skeletal muscle tissue lysates Lane 3: mouse heart tissue lysates Lane 4: mouse skeletal muscle tissue lysates Lane 5: human K562 whole cell lysates Lane 6: rat liver tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Desmin antigen affinity purified monoclonal antibody (Catalog # M01948-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Desmin at approximately 54KD. The expected band size for Desmin is at 54KD.
- Additional image
