MA5-15306

antibody from Invitrogen Antibodies

Targeting: DES CMD1I, CSM1, CSM2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15306

Provider

Invitrogen Antibodies

Product name

Desmin Monoclonal Antibody (10H7D2)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15306 targets Desmin in IHC and WB applications and shows reactivity with Human samples.

Antibody clone number

10H7D2

Concentration

1.0 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Desmin using Desmin monoclonal antibody (Product # MA5-15306) in HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Desmin cDNA (2).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Desmin Monoclonal Antibody (10H7D2) (Product # MA5-15306) and a 55 kDa band corresponding to Desmin was observed in Mouse Skeletal Muscle, Rat Skeletal Muscle, Mouse Heart, Rat Heart, Cardiomyocytes and RD cell line but not in Mouse Kidney, iPSC and MCF7 cell line. Two isoforms at ~40, ~50 kDa were also observed in Mouse Skeletal Muscle, Cardiomyocytes and RD cell line. An uncharacterized band of ~25 kDa was also observed in Mouse Skeletal Muscle and Mouse Heart. Tissue extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1), Rat Skeletal Muscle (Lane 2), Mouse Heart (Lane 3), Rat Heart (Lane 4), Mouse Kidney (Lane 7), whole cell extracts (30 µg lysate) of iPSC (Lane 5), Cardiomyocytes (Lane 6), RD (Lane 8) and MCF7 (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human smooth muscle sarcoma using Desmin monoclonal antibody (Product # MA5-15306) followed with DAB staining.