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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- MA5-16357 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Desmin Monoclonal Antibody (SP138)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is skeletal muscle tissue.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SP138
- Vial size
- 500 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Primary gastric choriocarcinoma in a dog.
Poutahidis T, Doulberis M, Karamanavi E, Angelopoulou K, Koutinas CK, Papazoglou LG
Journal of comparative pathology 2008 Aug-Oct;139(2-3):146-50
Journal of comparative pathology 2008 Aug-Oct;139(2-3):146-50
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Skeletal Muscle Cancer Tissue using anti-Desmin Monoclonal Antibody (Product # MA5-16357). The recommened dilution for this antibody in western blot applications is 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Desmin Monoclonal Antibody (SP138) (Product # MA5-16357) and a 55 kDa band corresponding to Desmin was observed in Mouse Skeletal Muscle, Rat Skeletal Muscle, Mouse Heart, Rat Heart, Cardiomyocytes and RD cell line but not in Mouse Kidney, iPSC and MCF7 cell line. Two isoforms at ~40, ~50 kDa were also observed in Mouse Skeletal Muscle, Cardiomyocytes and RD cell line. An uncharacterized band of ~25 kDa was also observed in Mouse Skeletal Muscle and Mouse Heart and ~110 kDa band in iPSC, RD and MCF7 cell lines. Tissue extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1), Rat Skeletal Muscle (Lane 2), Mouse Heart (Lane 3), Rat Heart (Lane 4), Mouse Kidney (Lane 7) and Whole cell extracts (30 µg lysate) of iPSC (Lane 5), Cardiomyocytes (Lane 6), RD (Lane 8) and MCF7 (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:100 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
