Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Immunohistochemistry [2]
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- Product number
- AF3844 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Desmin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse Desmin.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P17661
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Tumor stroma-targeted antibody-drug conjugate triggers localized anticancer drug release.
NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy.
Altered AKAP12 expression in portal fibroblasts and liver sinusoids mediates transition from hepatic fibrogenesis to fibrosis resolution.
Aneurysm Severity is Increased by Combined Mmp-7 Deletion and N-cadherin Mimetic (EC4-Fc) Over-Expression.
Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes.
Ang-2/VEGF bispecific antibody reprograms macrophages and resident microglia to anti-tumor phenotype and prolongs glioblastoma survival.
Dual inhibition of Ang-2 and VEGF receptors normalizes tumor vasculature and prolongs survival in glioblastoma by altering macrophages.
Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.
Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions.
The novel desmin mutant p.A120D impairs filament formation, prevents intercalated disk localization, and causes sudden cardiac death.
Dual color photoactivation localization microscopy of cardiomyopathy-associated desmin mutants.
Szot C, Saha S, Zhang XM, Zhu Z, Hilton MB, Morris K, Seaman S, Dunleavey JM, Hsu KS, Yu GJ, Morris H, Swing DA, Haines DC, Wang Y, Hwang J, Feng Y, Welsch D, DeCrescenzo G, Chaudhary A, Zudaire E, Dimitrov DS, St Croix B
The Journal of clinical investigation 2018 Jul 2;128(7):2927-2943
The Journal of clinical investigation 2018 Jul 2;128(7):2927-2943
NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy.
Dubrac A, Künzel SE, Künzel SH, Li J, Chandran RR, Martin K, Greif DM, Adams RH, Eichmann A
Nature communications 2018 Aug 27;9(1):3463
Nature communications 2018 Aug 27;9(1):3463
Altered AKAP12 expression in portal fibroblasts and liver sinusoids mediates transition from hepatic fibrogenesis to fibrosis resolution.
Lee HS, Choi J, Son T, Wee HJ, Bae SJ, Seo JH, Park JH, Ryu SH, Lee D, Jang MK, Yu E, Chung YH, Kim KW
Experimental & molecular medicine 2018 Apr 27;50(4):48
Experimental & molecular medicine 2018 Apr 27;50(4):48
Aneurysm Severity is Increased by Combined Mmp-7 Deletion and N-cadherin Mimetic (EC4-Fc) Over-Expression.
Lyon CA, Williams H, Bianco R, Simmonds SJ, Brown BA, Wadey KS, Smith FCT, Johnson JL, George SJ
Scientific reports 2017 Dec 11;7(1):17342
Scientific reports 2017 Dec 11;7(1):17342
Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes.
Liu Q, Yang R, Huang X, Zhang H, He L, Zhang L, Tian X, Nie Y, Hu S, Yan Y, Zhang L, Qiao Z, Wang QD, Lui KO, Zhou B
Cell research 2016 Jan;26(1):119-30
Cell research 2016 Jan;26(1):119-30
Ang-2/VEGF bispecific antibody reprograms macrophages and resident microglia to anti-tumor phenotype and prolongs glioblastoma survival.
Kloepper J, Riedemann L, Amoozgar Z, Seano G, Susek K, Yu V, Dalvie N, Amelung RL, Datta M, Song JW, Askoxylakis V, Taylor JW, Lu-Emerson C, Batista A, Kirkpatrick ND, Jung K, Snuderl M, Muzikansky A, Stubenrauch KG, Krieter O, Wakimoto H, Xu L, Munn LL, Duda DG, Fukumura D, Batchelor TT, Jain RK
Proceedings of the National Academy of Sciences of the United States of America 2016 Apr 19;113(16):4476-81
Proceedings of the National Academy of Sciences of the United States of America 2016 Apr 19;113(16):4476-81
Dual inhibition of Ang-2 and VEGF receptors normalizes tumor vasculature and prolongs survival in glioblastoma by altering macrophages.
Peterson TE, Kirkpatrick ND, Huang Y, Farrar CT, Marijt KA, Kloepper J, Datta M, Amoozgar Z, Seano G, Jung K, Kamoun WS, Vardam T, Snuderl M, Goveia J, Chatterjee S, Batista A, Muzikansky A, Leow CC, Xu L, Batchelor TT, Duda DG, Fukumura D, Jain RK
Proceedings of the National Academy of Sciences of the United States of America 2016 Apr 19;113(16):4470-5
Proceedings of the National Academy of Sciences of the United States of America 2016 Apr 19;113(16):4470-5
Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
Riordan DP, Varma S, West RB, Brown PO
PloS one 2015;10(7):e0128975
PloS one 2015;10(7):e0128975
Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.
Maury Y, Côme J, Piskorowski RA, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S
Nature biotechnology 2015 Jan;33(1):89-96
Nature biotechnology 2015 Jan;33(1):89-96
Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions.
Durruthy-Durruthy J, Briggs SF, Awe J, Ramathal CY, Karumbayaram S, Lee PC, Heidmann JD, Clark A, Karakikes I, Loh KM, Wu JC, Hoffman AR, Byrne J, Reijo Pera RA, Sebastiano V
PloS one 2014;9(4):e94231
PloS one 2014;9(4):e94231
The novel desmin mutant p.A120D impairs filament formation, prevents intercalated disk localization, and causes sudden cardiac death.
Brodehl A, Dieding M, Klauke B, Dec E, Madaan S, Huang T, Gargus J, Fatima A, Saric T, Cakar H, Walhorn V, Tönsing K, Skrzipczyk T, Cebulla R, Gerdes D, Schulz U, Gummert J, Svendsen JH, Olesen MS, Anselmetti D, Christensen AH, Kimonis V, Milting H
Circulation. Cardiovascular genetics 2013 Dec;6(6):615-23
Circulation. Cardiovascular genetics 2013 Dec;6(6):615-23
Dual color photoactivation localization microscopy of cardiomyopathy-associated desmin mutants.
Brodehl A, Hedde PN, Dieding M, Fatima A, Walhorn V, Gayda S, Šarić T, Klauke B, Gummert J, Anselmetti D, Heilemann M, Nienhaus GU, Milting H
The Journal of biological chemistry 2012 May 4;287(19):16047-57
The Journal of biological chemistry 2012 May 4;287(19):16047-57
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Desmin in Human Heart. Desmin was detected in immersion fixed paraffin-embedded sections of human heart using 15 µg/mL Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Desmin in Mouse Skeletal Muscle. Desmin was detected in immersion fixed frozen sections of mouse skeletal muscle using Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to z-lines. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.