15370-1-AP

antibody from Proteintech Group

Targeting: NFS1 IscS, NifS

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

15370-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#15370-1-AP, RRID:AB_2151933

Product name

NFS1 antibody

Antibody type

Polyclonal

Description

KD/KO validated NFS1 antibody (Cat. #15370-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

NFS1 Regulates IDH2 to Attenuate Abdominal Aortic Aneurysms via Interacting With SP2.

Zhang L, Zhang Y, Wen D, Guo S, Qi X, Wang H, Sun Y, Yang G, Wang Y, Xue S
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Mar;13(14):e05240

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Succinylation modification on NFS1 ameliorates the glucose starvation-induced ferroptosis through maintaining mitochondrial iron homeostasis in oral squamous cell carcinoma cells.

Ling H, Qing L, Zuo L, Wang H, Cai X, Liu Y, Tian H
Biochimica et biophysica acta. Molecular basis of disease 2026 Apr 23;1872(6):168276

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NFS1, together with FXN, protects cells from ferroptosis and DNA damage in diffuse large B-cell lymphoma.

Shi X, Zhao Y, Gao HY, Yang W, Liao J, Wang HH, Wang XT, Yan W
Redox biology 2025 Nov;87:103878

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Impaired Iron-Sulfur Cluster Synthesis Induces Mitochondrial PARthanatos in Diabetic Cardiomyopathy.

Wang M, Zhang S, Tian J, Yang F, Chen H, Bai S, Kang J, Pang K, Huang J, Dong M, Dong S, Tian Z, Fang S, Fan H, Lu F, Yu B, Li S, Zhang W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jan;12(1):e2406695

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Downregulation of the Zinc Transporter ZIP13 (Slc39a13) Leads to Ferroptosis by Inhibiting Mitochondrial Iron-Sulfur Cluster Biosynthesis and Induces Ischemia/Reperfusion Injury in Mouse Hearts.

Zhang R, Wang J, Yang Q, Yu Y, Cheng X, Xu Z
Antioxidants & redox signaling 2025 Aug;43(4-6):328-344

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Histone lactylation drives liver cancer metastasis by facilitating NSF1-mediated ferroptosis resistance after microwave ablation.

Huang J, Xie H, Li J, Huang X, Cai Y, Yang R, Yang D, Bao W, Zhou Y, Li T, Lu Q
Redox biology 2025 Apr;81:103553

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Overexpression of Nfs1 Cysteine Desulphurase Relieves Sevoflurane-Induced Neurotoxicity and Cognitive Dysfunction in Neonatal Mice Via Suppressing Oxidative Stress and Ferroptosis.

Zhang Y, Liu X, Xie L, Hong J, Zhuang Q, Ren L, Li X, Zhang C
Journal of biochemical and molecular toxicology 2024 Nov;38(11):e70051

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Downregulation of Iron-Sulfur Cluster Biogenesis May Contribute to Hyperglycemia-Mediated Diabetic Peripheral Neuropathy in Murine Models.

Wu L, Huang F, Sun Z, Zhang J, Xia S, Zhao H, Liu Y, Yang L, Ding Y, Bian D, Li K, Sun Y
Antioxidants (Basel, Switzerland) 2024 Aug 26;13(9)

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L-cysteine contributes to destructive activities of odontogenic cysts/tumor.

Li J, Feng C, Pang X, Li X, Dou X, Jiang E, Shang Z
Discover oncology 2024 Apr 8;15(1):109

---

Ferroptosis Inducers Kill Mesenchymal Stem Cells Affected by Neuroblastoma.

Li X, Wang Q, Xu C, Zhang L, Zhou J, Lv J, Xu M, Jiang D
Cancers 2023 Feb 18;15(4)

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Cur@SF NPs alleviate Friedreich's ataxia in a mouse model through synergistic iron chelation and antioxidation.

Xu L, Sun Z, Xing Z, Liu Y, Zhao H, Tang Z, Luo Y, Hao S, Li K
Journal of nanobiotechnology 2022 Mar 9;20(1):118

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NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.

Hou T, Zhang R, Jian C, Ding W, Wang Y, Ling S, Ma Q, Hu X, Cheng H, Wang X
Cell research 2019 Sep;29(9):754-766

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4'-Phosphopantetheine and long acyl chain-dependent interactions are integral to human mitochondrial acyl carrier protein function.

Majmudar JD, Feng X, Fox NG, Nabhan JF, Towle T, Ma T, Gooch R, Bulawa C, Yue WW, Martelli A
MedChemComm 2019 Feb 1;10(2):209-220

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NDUFAB1 protects against obesity and insulin resistance by enhancing mitochondrial metabolism.

Zhang R, Hou T, Cheng H, Wang X
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Dec;33(12):13310-13322

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Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.

Ferecatu I, Canal F, Fabbri L, Mazure NM, Bouton C, Golinelli-Cohen MP
PloS one 2018;13(3):e0194782

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 15370-1-AP(NFS1 antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using NFS1 antibody 15370-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human heart using 15370-1-AP(NFS1 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human heart using 15370-1-AP(NFS1 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue