Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [6]
- Immunohistochemistry [2]
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- Product number
- PA5-117064 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: SH-SY5Y, HepG2, BxPC-3, Mouse brain, Mouse liver, Mouse heart, Rat brain, Rat liver, Rat heart Immunogen sequence: GMRSGTVPTP LVVGLGAACE VAQQEMEYDH KRISKLSERL IQNIMKSLPD VVMNGDPKHH YPGCINLSFA YVEGESLLMA LKDVALSSGS ACTSASLEPS YVLRAIGTDE DLAHSSIRFG IGRFTTEEEV DYTVEKCIQH VKRLREMSPL WEMVQDGIDL KSIKWTQH
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.07 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFS1 in extracts of various cell lines. Samples were incubated with NFS1 Polyclonal antibody (Product # PA5-117064) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NFS1 in L929 cells. Samples were incubated with NFS1 Polyclonal antibody (Product # PA5-117064) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of NFS1 in C6 cells using NFS1 Polyclonal Antibody (Product # PA5-117064) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of NFS1 in L929 cells using NFS1 Polyclonal Antibody (Product # PA5-117064) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of NFS1 in U-251 MG cells using NFS1 Polyclonal Antibody (Product # PA5-117064) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NFS1 in U-251 MG cells. Samples were incubated with NFS1 Polyclonal antibody (Product # PA5-117064) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NFS1 in C6 cells. Samples were incubated with NFS1 Polyclonal antibody (Product # PA5-117064) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NFS1 in paraffin-embedded rat brain. Samples were incubated with NFS1 Polyclonal antibody (Product # PA5-117064) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NFS1 in paraffin-embedded rat brain. Samples were incubated with NFS1 Polyclonal antibody (Product # PA5-117064) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.