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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00004830-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004830-M02, RRID:AB_546522
- Product name
- Mouse Monoclonal NM23-H1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. NME1 - non-metastatic cells 1, protein (NM23A) expressed in
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references A transcriptome-based protein network that identifies new therapeutic targets in colorectal cancer.
Identification of antigenic proteins associated with trichloroethylene-induced autoimmune disease by serological proteome analysis.
Durand S, Trillet K, Uguen A, Saint-Pierre A, Le Jossic-Corcos C, Corcos L
BMC genomics 2017 Sep 30;18(1):758
BMC genomics 2017 Sep 30;18(1):758
Identification of antigenic proteins associated with trichloroethylene-induced autoimmune disease by serological proteome analysis.
Liu J, Xing X, Huang H, Jiang Y, He H, Xu X, Yuan J, Zhou L, Yang L, Zhuang Z
Toxicology and applied pharmacology 2009 Nov 1;240(3):393-400
Toxicology and applied pharmacology 2009 Nov 1;240(3):393-400
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody (M02), clone 1D7.Lane 1: NME1 transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - NME1 monoclonal antibody (M02), clone 1D7. Analysis of NME1 expression in different cell lines.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - NME1 monoclonal antibody (M02), clone 1D7. Analysis of NME1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: NM23-H1 Antibody (1D7) [H00004830-M02] - Detection limit for recombinant GST tagged NME1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 transfected lysate using anti-NME1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NME1 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
