Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Western blot [4]
 - Immunocytochemistry [1]
 - Immunohistochemistry [3]
 - Other assay [1]
 
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Validation data
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- Product number
 - 11086-2-AP - Provider product page

 - Provider
 - Invitrogen Antibodies
 - Product name
 - NME1 Polyclonal Antibody
 - Antibody type
 - Polyclonal
 - Antigen
 - Other
 - Reactivity
 - Human
 - Host
 - Rabbit
 - Isotype
 - IgG
 - Vial size
 - 150 µL
 - Concentration
 - 0.35 mg/mL
 - Storage
 - -20°C
 
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					Supportive validation
					
									
				
				- Submitted by
 - Invitrogen Antibodies (provider)
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- Experimental details
 - WB result of 10086-2-AP (NME1 antibody).
 
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 - Invitrogen Antibodies (provider)
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- Experimental details
 - MCF7 cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
 
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 - Invitrogen Antibodies (provider)
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- Experimental details
 - HeLa cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
 
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 - Invitrogen Antibodies (provider)
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- Experimental details
 - A549 cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
 
							
					Supportive validation
					
									
				
				- Submitted by
 - Invitrogen Antibodies (provider)
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- Experimental details
 - Immunofluorescent analysis of MCF-7 cells using 11086-2-AP (NME1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
 
							
					Supportive validation
					
									
				
				- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human pancreas cancer using 11086-2-AP (NME1 antibody) at dilution of 1:100 (under 25x lens).
 
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 - Invitrogen Antibodies (provider)
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- Experimental details
 - Immunohistochemistry of paraffin-embedded human lung cancer using 11086-2-AP (NME1 antibody) at dilution of 1:100 (under 10x lens).
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human lung cancer using 11086-2-AP (NME1 antibody) at dilution of 1:100 (under 40x lens).
 
							
					Supportive validation
					
									
				
		- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - IP result of anti-NME1 (IP:11086-2-AP, 4ug; Detection:11086-2-AP 1:500) with A549 cells lysate 2800ug.