MA5-15642

antibody from Invitrogen Antibodies

Targeting: NME1 NDPKA, NM23, NM23-H1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15642

Provider

Invitrogen Antibodies

Product name

NME1 Monoclonal Antibody (4B2)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15642 targets NME1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15642 immunogen is purified recombinant fragment of human NME1 expressed in E. Coli.. MA5-15642 detects NME1 which has a predicted molecular weight of approximately 17kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4B2

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-NME1 Monoclonal Antibody (Product # MA5-15642) and a 17 kDa band corresponding to NME1 was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), PC-3 (Lane 3) and Raji (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NME1 using NME1 monoclonal antibody (Product # MA5-15642) in NME1 (AA: 27-177) human IgG Fc transfected HEK293 cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using NME1 monoclonal antibody (Product # MA5-15642) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using NME1 monoclonal antibody (Product # MA5-15642) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using NME1 monoclonal antibody (Product # MA5-15642) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using NME1 monoclonal antibody (Product # MA5-15642) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human placenta tissues using NME1 monoclonal antibody (Product # MA5-15642).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells using NME1 monoclonal antibody (Product # MA5-15642) (green) and negative control (purple).