PA1-9565

antibody from Invitrogen Antibodies

Targeting: GFAP FLJ45472

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-9565

Provider

Invitrogen Antibodies

Product name

GFAP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

PA1-9565 detects GFAP in human, mouse and rat samples.

Concentration

1 mg/mL

References

Submitted references

Microfluidic Multielectrode Arrays for Spatially Localized Drug Delivery and Electrical Recordings of Primary Neuronal Cultures.

Bruno G, Colistra N, Melle G, Cerea A, Hubarevich A, Deleye L, De Angelis F, Dipalo M
Frontiers in bioengineering and biotechnology 2020;8:626

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Excessive Astrocytic GABA Causes Cortical Hypometabolism and Impedes Functional Recovery after Subcortical Stroke.

Nam MH, Cho J, Kwon DH, Park JY, Woo J, Lee JM, Lee S, Ko HY, Won W, Kim RG, Song H, Oh SJ, Choi JW, Park KD, Park EK, Jung H, Kim HS, Lee MC, Yun M, Lee CJ, Kim HI
Cell reports 2020 Jul 7;32(1):107861

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Amacrine cells coupled to ganglion cells via gap junctions are highly vulnerable in glaucomatous mouse retinas.

Akopian A, Kumar S, Ramakrishnan H, Viswanathan S, Bloomfield SA
The Journal of comparative neurology 2019 Jan 1;527(1):159-173

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Age-related changes in the optic nerve of Sprague-Dawley rats: an ultrastructural and immunohistochemical study.

Yassa HD
Acta histochemica 2014 Jul;116(6):1085-95

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Fasudil protects hippocampal neurons against hypoxia-reoxygenation injury by suppressing microglial inflammatory responses in mice.

Ding J, Li QY, Wang X, Sun CH, Lu CZ, Xiao BG
Journal of neurochemistry 2010 Sep;114(6):1619-29

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-GFAP Rabbit Polyclonal Antibody (Product # PA1-9565) and a 50 kDa band corresponding to GFAP was observed across tissues tested except Mouse and Rat liver. Whole cell extracts (30 µg lysate) of Mouse brain (Lane 1), Rat brain (Lane 2), Mouse liver (Lane 3) and Rat liver (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Intracameral injection of microbeads elevates IOP of mouse eyes. A : An initial injection of microbeads followed by a second injection at 4 weeks (arrow) resulted in a significant elevation of IOP that was maintained for at least 8 weeks (red circles). Mice that received PBS sham injections showed no change in IOP from steady values at week 0 (black circles). B : Elevated IOP reduced the thickness of the entire retina as well as the individual inner nuclear layer (INL), inner plexiform layer (IPL), and ganglion cell layer (GCL). C : In control retinas, GFAP immunofluorescence was confined almost exclusively to astrocytes in the GCL. D : At 8 weeks after initial microbead injection, the GFAP labeling was dramatically increased due to expanded expression in Muller cell processes extending across retinal layers. E : Histograms quantify and compare the mean percentage area of GFAP labeling in control and glaucomatous retinas. Measurements were made of fluorescent pixels within a 100- x 100-mum square (in C and D ). Three to four area measurements were made and averaged in five control and five microbead-injected retinas. F : The fluorescent intensity profile of GFAP labeling for pixels measured along a single line profile (white lines in C and D ) shows its upregulation in the IPL and INL of glaucomatous retinas compared with controls, with the largest increase in expression in the IPL. Bars indicate mean value +- SD (in A) and mean +- SEM (applies to B and E). *, P