HPA056030

antibody from Atlas Antibodies

Targeting: GFAP FLJ45472

Provider product page for HPA056030

Western blot

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [36]
Comments [0]
Validations
Immunohistochemistry [1]

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Product number: HPA056030 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA056030, RRID:AB_2683015
Product name: Anti-GFAP
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human GFAP, Gene description: glial fibrillary acidic protein, Alternative Gene Names: FLJ45472, Validated applications: WB, IHC, ICC, Uniprot ID: , Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.1 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Handling: The antibody solution should be gently mixed before use.

GFAP protein structure - HPA056030 shown in red.

Submitted references Brain-derived endothelial cells are neuroprotective in a chronic cerebral hypoperfusion mouse model

Matsui Y, Muramatsu F, Nakamura H, Noda Y, Matsumoto K, Kishima H, Takakura N
Communications Biology 2024;7(1)

ATM-deficiency-induced microglial activation promotes neurodegeneration in ataxia-telangiectasia

Lai J, Demirbas D, Kim J, Jeffries A, Tolles A, Park J, Chittenden T, Buckley P, Yu T, Lodato M, Lee E
Cell Reports 2024;43(1):113622

Caveolin-1 mediates blood-brain barrier permeability, neuroinflammation, and cognitive impairment in SARS-CoV-2 infection

Trevino T, Almousawi A, Robinson K, Fogel A, Class J, Minshall R, Tai L, Richner J, Lutz S
Journal of Neuroimmunology 2024;388

Patient-derived induced pluripotent stem cells with a MERTK mutation exhibit cell junction abnormalities and aberrant cellular differentiation potential

Zhang H, Wu L, Liu Z, Jin Z
World Journal of Stem Cells 2024;16(5):512-524

Optochemical control of slow-wave sleep in the nucleus accumbens of male mice by a photoactivatable allosteric modulator of adenosine A2A receptors

Roy K, Zhou X, Otani R, Yuan P, Ioka S, Vogt K, Kondo T, Farag N, Ijiri H, Wu Z, Chitose Y, Amezawa M, Uygun D, Cherasse Y, Nagase H, Li Y, Yanagisawa M, Abe M, Basheer R, Wang Y, Saitoh T, Lazarus M
Nature Communications 2024;15(1)

Nanoparticle-based inhibition of vascular endothelial growth factor receptors alleviates osteoarthritis pain and cartilage damage

Ma K, Pham T, Wang J, O-Sullivan I, DiCamillo A, Du S, Mwale F, Farooqui Z, Votta-Velis G, Bruce B, van Wijnen A, Liu Y, Im H
Science Advances 2024;10(7)

Semaglutide Ameliorates Diabetic Neuropathic Pain by Inhibiting Neuroinflammation in the Spinal Cord.

Lee SO, Kuthati Y, Huang WH, Wong CS
Cells 2024 Nov 8;13(22)

No Evidence of Sensory Neuropathy in a Traditional Mouse Model of Idiopathic Parkinson's Disease.

Faisal M, Rusetskaya A, Väli L, Taba P, Minajeva A, Hickey MA
Cells 2024 May 8;13(10)

The Melatonin Type 2 Receptor Agonist IIK7 Attenuates and Reverses Morphine Tolerance in Neuropathic Pain Rats Through the Suppression of Neuroinflammation in the Spinal Cord.

Kuthati Y, Wong CS
Pharmaceuticals (Basel, Switzerland) 2024 Dec 5;17(12)

Spinal cord injury-induced gut dysbiosis influences neurological recovery partly through short-chain fatty acids

Jing Y, Yang D, Bai F, Wang Q, Zhang C, Yan Y, Li Z, Li Y, Chen Z, Li J, Yu Y
npj Biofilms and Microbiomes 2023;9(1)

Astrocytic β-catenin signaling via TCF7L2 regulates synapse development and social behavior

Szewczyk L, Lipiec M, Liszewska E, Meyza K, Urban-Ciecko J, Kondrakiewicz L, Goncerzewicz A, Rafalko K, Krawczyk T, Bogaj K, Vainchtein I, Nakao-Inoue H, Puscian A, Knapska E, Sanders S, Jan Nowakowski T, Molofsky A, Wisniewska M
Molecular Psychiatry 2023;29(1):57-73

Glial cells are affected more than interneurons by the loss of Engrailed 2 gene in the mouse cerebellum

Lazzarini G, Gatta A, Miragliotta V, Vaglini F, Viaggi C, Pirone A
Journal of Anatomy 2023;244(4):667-675

Patchouli alcohol attenuates the cognitive deficits in a transgenic mouse model of Alzheimer’s disease via modulating neuropathology and gut microbiota through suppressing C/EBPβ/AEP pathway

Xu Q, Su Z, Yang W, Zhong M, Xian Y, Lin Z
Journal of Neuroinflammation 2023;20(1)

Targeting Vascular Endothelial Growth Factor Receptors as a Therapeutic Strategy for Osteoarthritis and Associated Pain

Ma K, Singh G, Wang J, O-Sullivan I, Votta-Velis G, Bruce B, Anbazhagan A, van Wijnen A, Im H
International Journal of Biological Sciences 2023;19(2):675-690

The neuroprotective potential of mesenchymal stem cells from bone marrow and human exfoliated deciduous teeth in a murine model of demyelination

Forsthuber T, Kråkenes T, Wergeland S, Al-Sharabi N, Mohamed-Ahmed S, Fromreide S, Costea D, Mustafa K, Bø L, Kvistad C
PLOS ONE 2023;18(11):e0293908

CRX haploinsufficiency compromises photoreceptor precursor translocation and differentiation in human retinal organoids

Pan D, Zhang X, Jin K, Jin Z
Stem Cell Research & Therapy 2023;14(1)

Nano-Honokiol ameliorates the cognitive deficits in TgCRND8 mice of Alzheimer’s disease via inhibiting neuropathology and modulating gut microbiota

Qu C, Li Q, Su Z, Ip S, Yuan Q, Xie Y, Xu Q, Yang W, Huang Y, Xian Y, Lin Z
Journal of Advanced Research 2022;35

PEG-mediated transduction of rAAV as a platform for spatially confined and efficient gene delivery

Zou L, Wang J, Fang Y, Tian H
Biomaterials Research 2022;26(1)

Bidirectional Modulation of Neuronal Cells Electrical and Mechanical Properties Through Pristine and Functionalized Graphene Substrates

Zummo F, Esposito P, Hou H, Wetzl C, Rius G, Tkatchenko R, Guimera A, Godignon P, Prato M, Prats-Alfonso E, Criado A, Scaini D
Frontiers in Neuroscience 2022;15

Micro-RNAs Shuttled by Extracellular Vesicles Secreted from Mesenchymal Stem Cells Dampen Astrocyte Pathological Activation and Support Neuroprotection in In-Vitro Models of ALS

Provenzano F, Nyberg S, Giunti D, Torazza C, Parodi B, Bonifacino T, Usai C, Kerlero de Rosbo N, Milanese M, Uccelli A, Shaw P, Ferraiuolo L, Bonanno G
Cells 2022;11(23):3923

Fecal Microbiota Transplantation Exerts Neuroprotective Effects in a Mouse Spinal Cord Injury Model by Modulating the Microenvironment at the Lesion Site

Jing Y, Bai F, Wang L, Yang D, Yan Y, Wang Q, Zhu Y, Yu Y, Chen Z, Xu Z
Microbiology Spectrum 2022;10(3)

Optogenetic induction of hibernation-like state with modified human Opsin4 in mice.

Takahashi TM, Hirano A, Kanda T, Saito VM, Ashitomi H, Tanaka KZ, Yokoshiki Y, Masuda K, Yanagisawa M, Vogt KE, Tokuda T, Sakurai T
Cell reports methods 2022 Nov 21;2(11):100336

Cold Atmospheric Plasma Does Not Affect Stellate Cells Phenotype in Pancreatic Cancer Tissue in Ovo.

Privat-Maldonado A, Verloy R, Cardenas Delahoz E, Lin A, Vanlanduit S, Smits E, Bogaerts A
International journal of molecular sciences 2022 Feb 10;23(4)

Adropin correlates with aging-related neuropathology in humans and improves cognitive function in aging mice

Banerjee S, Ghoshal S, Girardet C, DeMars K, Yang C, Niehoff M, Nguyen A, Jayanth P, Hoelscher B, Xu F, Banks W, Hansen K, Zhang J, Candelario-Jalil E, Farr S, Butler A
npj Aging and Mechanisms of Disease 2021;7(1)

High-fat diet impairs duodenal barrier function and elicits glia-dependent changes along the gut-brain axis that are required for anxiogenic and depressive-like behaviors

Seguella L, Pesce M, Capuano R, Casano F, Pesce M, Corpetti C, Vincenzi M, Maftei D, Lattanzi R, Del Re A, Sarnelli G, Gulbransen B, Esposito G
Journal of Neuroinflammation 2021;18(1)

Effect ofAPOEε4 allele on levels of apolipoproteins E, J, and D, and redox signature in circulating extracellular vesicles from cognitively impaired with no dementia participants converted to Alzheimer's disease

Ben Khedher M, Haddad M, Laurin D, Ramassamy C
Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring 2021;13(1)

Self-assembled multifunctional neural probes for precise integration of optogenetics and electrophysiology

Zou L, Tian H, Guan S, Ding J, Gao L, Wang J, Fang Y
Nature Communications 2021;12(1)

Patient-Specific Retinal Organoids Recapitulate Disease Features of Late-Onset Retinitis Pigmentosa

Gao M, Lei X, Han F, He K, Jin S, Zhang Y, Jin Z
Frontiers in Cell and Developmental Biology 2020;8

Nitrosative Stress in the Frontal Cortex From Dogs With Canine Cognitive Dysfunction

Prpar Mihevc S, Zakošek Pipan M, Štrbenc M, Rogelj B, Majdič G
Frontiers in Veterinary Science 2020;7

Sulforaphene Ameliorates Neuroinflammation and Hyperphosphorylated Tau Protein via Regulating the PI3K/Akt/GSK-3βPathway in Experimental Models of Alzheimer’s Disease

Yang W, Liu Y, Xu Q, Xian Y, Lin Z
Oxidative Medicine and Cellular Longevity 2020;2020

A Toolbox of Criteria for Distinguishing Cajal-Retzius Cells from Other Neuronal Types in the Postnatal Mouse Hippocampus.

Anstötz M, Maccaferri G
eNeuro 2020 Jan Feb;7(1)

Plasma protein profiling reveals candidate biomarkers for multiple sclerosis treatment.

Bedri SK, Nilsson OB, Fink K, Månberg A, Hamsten C, Ayoglu B, Manouchehrinia A, Nilsson P, Olsson T, Hillert J, Grönlund H, Glaser A
PloS one 2019;14(5):e0217208

EGR1 recruits TET1 to shape the brain methylome during development and upon neuronal activity

Sun Z, Xu X, He J, Murray A, Sun M, Wei X, Wang X, McCoig E, Xie E, Jiang X, Li L, Zhu J, Chen J, Morozov A, Pickrell A, Theus M, Xie H
Nature Communications 2019;10(1)

MicroRNA-365 alleviates morphine analgesic tolerance via the inactivation of the ERK/CREB signaling pathway by negatively targeting β-arrestin2

Wu X, She R, Yang Y, Xing Z, Chen H, Zhang Y
Journal of Biomedical Science 2018;25(1)

Protective effects of primary neural stem cell treatment in ischemic stroke models

Yu X, Wang X, Zeng S, Tuo X
Experimental and Therapeutic Medicine 2018

Clinicoradiological characteristics, management and prognosis of primary myeloid sarcoma of the central nervous system: A report of four cases

Yang B, Yang C, Fang J, Yang J, Xu Y
Oncology Letters 2017;14(3):3825-3831

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using HPA056030 antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.
Sample type: Human
Protocol: Protocol
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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