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- Western blot [1]
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- Product number
- ABIN1842219 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Glial Fibrillary Acidic Protein (GFAP) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Rat, Bovine, Porcine
- Host
- Chicken/Avian
- Isotype
- IgY
- Vial size
- 100 μL
Submitted references Tobacco smoke diminishes neurogenesis and promotes gliogenesis in the dentate gyrus of adolescent rats.
Bruijnzeel AW, Bauzo RM, Munikoti V, Rodrick GB, Yamada H, Fornal CA, Ormerod BK, Jacobs BL
Brain research 2011 Sep 21;1413:32-42
Brain research 2011 Sep 21;1413:32-42
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- Experimental details
- Western blots of crude whole tissue homogenates of adult rat cortex, cerebellum, brain stem and spinal cord. 100μg of wet weight of tissue loaded per lane, electrophoretically transferred to nitrocellulose and blotted with RPCA-GFAP, used at a dilution of 1:100,000. Examples: Check http://www.bio.com/Album/Chickenabpics.htm for pictures made with this antibody. References: 1. Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 43:429-35 1972. 2. Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE and Messing A. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet 27:117-20 2001 3. Liem RKH, Yen SH, Salomon GD and Shelanski ML. Intermediate filaments in nervous tissues. J Cell Biol 79:637-745 (1978). 4. Bruijnzeel AW, Bauzo RM, Munikoti V, Rodrick GB, Yamada H, Fornal CA, Ormerod BK, Jacobs BL.Tobacco smoke diminishes neurogenesis and promotes gliogenesis in the dentate gyrus of adolescent rats. Brain Res. 1413:32-42 (2011). Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. © Biotechnology Inc. May 23, 2014.
