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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AM1870b - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1870b, RRID:AB_10814150
- Product name
- GFAP Antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant Protein
- Description
- Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- GFAP Antibody (Cat. #AM1870b) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the GFAP antibody detected the GFAP protein (arrow).
- Primary Ab dilution
- 1:100~500
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of GFAP Antibody (Cat#AM1870b) with brain tissue followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- GFAP Monoclonal antibody (Cat. #AM1870b) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFAP Monoclonal antibody( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:50~100
