Antibody data
- Antibody Data
- Antigen structure
- References [66]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- PB9082 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-GFAP Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for GFAP detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. GFAP information: Molecular Weight: 49880 MW; Subcellular Localization: Cytoplasm . Associated with intermediate filaments; Tissue Specificity: Expressed in cells lacking fibronectin.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Sevoflurane Alters Serum Metabolites in Elders and Aging Mice and Increases Inflammation in Hippocampus.
Transplantation of Wnt5a-modified Bone Marrow Mesenchymal Stem Cells Promotes Recovery After Spinal Cord Injury via the PI3K/AKT Pathway.
Menthol-Modified Quercetin Liposomes with Brain-Targeting Function for the Treatment of Senescent Alzheimer's Disease.
Tauroursodeoxycholic Acid Inhibited Apoptosis and Oxidative Stress in H(2)O(2)-Induced BMSC Death via Modulating the Nrf-2 Signaling Pathway: the Therapeutic Implications in a Rat Model of Spinal Cord Injury.
Transcriptional and epigenetic dysregulation impairs generation of proliferative neural stem and progenitor cells during brain aging.
Borneol-Modified Schisandrin B Micelles Cross the Blood-Brain Barrier To Treat Alzheimer's Disease in Aged Mice.
Transplantation of MiR-28-5p-Modified BMSCs Promotes Functional Recovery After Spinal Cord Injury.
Vagus Nerve Stimulation Prevents Endothelial Necroptosis to Alleviate Blood-Spinal Cord Barrier Disruption After Spinal Cord Injury.
Micro electrical fields induced MSC-sEVs attenuate neuronal cell apoptosis by activating autophagy via lncRNA MALAT1/miR-22-3p/SIRT1/AMPK axis in spinal cord injury.
Measurement of Patient-Derived Glioblastoma Cell Response to Temozolomide Using Fluorescence Lifetime Imaging of NAD(P)H.
Sex Differences in the Expression of Neuroimmune Molecules in the Spinal Cord of a Mouse Model of Antiretroviral-Induced Neuropathic Pain.
Upregulation of SLITRK5 in patients with epilepsy and in a rat model.
A reactive oxygen species-responsive hydrogel encapsulated with bone marrow derived stem cells promotes repair and regeneration of spinal cord injury.
Engineered exosomes enriched in netrin-1 modRNA promote axonal growth in spinal cord injury by attenuating inflammation and pyroptosis.
Nanozyme-Integrated Thermoresponsive In Situ Forming Hydrogel Enhances Mesenchymal Stem Cell Viability and Paracrine Effect for Efficient Spinal Cord Repair.
Engineering of M2 Macrophages-Derived Exosomes via Click Chemistry for Spinal Cord Injury Repair.
Vagus Nerve Stimulation Reduces Neuroinflammation Through Microglia Polarization Regulation to Improve Functional Recovery After Spinal Cord Injury.
Mechanobiological responses of astrocytes in optic nerve head due to biaxial stretch.
Olfactory Ensheathing Cells Alleviate Facial Pain in Rats with Trigeminal Neuralgia by Inhibiting the Expression of P2X7 Receptor.
Hypoxia-stimulated mesenchymal stem cell-derived exosomes loaded by adhesive hydrogel for effective angiogenic treatment of spinal cord injury.
EphA4 Obstructs Spinal Cord Neuron Regeneration by Promoting Excessive Activation of Astrocytes.
Stimulation by Exosomes from Hypoxia Preconditioned Human Umbilical Vein Endothelial Cells Facilitates Mesenchymal Stem Cells Angiogenic Function for Spinal Cord Repair.
Bu Shen Huo Xue decoction promotes functional recovery in spinal cord injury mice by improving the microenvironment to promote axonal regeneration.
Electroacupuncture alleviates spatial memory deficits in METH withdrawal mice by enhancing astrocyte-mediated glutamate clearance in the dCA1.
Inhibition of neuronal nitric oxide synthase protects against hippocampal neuronal injuries by increasing neuropeptide Y expression in temporal lobe epilepsy mice.
Astrocyte-selective STAT3 knockdown rescues methamphetamine withdrawal-disrupted spatial memory in mice via restoring the astrocytic capacity of glutamate clearance in dCA1.
Tak1 in the astrocytes of mediobasal hypothalamus regulates anxiety-like behavior in mice.
Modulatory Potential of LncRNA Zfas1 for Inflammation and Neuronal Apoptosis in Temporal Lobe Epilepsy.
Effect of electroacupuncture on glial fibrillary acidic protein and nerve growth factor in the hippocampus of rats with hyperlipidemia and middle cerebral artery thrombus.
Maternal Benzophenone Exposure Impairs Hippocampus Development and Cognitive Function in Mouse Offspring.
Klotho ameliorated cognitive deficits in a temporal lobe epilepsy rat model by inhibiting ferroptosis.
Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model.
Expression and cellular distribution of FGF13 in cortical tubers of the tuberous sclerosis complex.
Dynamic-related protein 1 inhibitor eases epileptic seizures and can regulate equilibrative nucleoside transporter 1 expression.
α7nAchR mediates transcutaneous auricular vagus nerve stimulation-induced neuroprotection in a rat model of ischemic stroke by enhancing axonal plasticity.
Renal and cerebral RAS interaction contributes to diabetic kidney disease.
Biofunctionalized self-assembly of peptide amphiphile induces the differentiation of bone marrow mesenchymal stem cells into neural cells.
Structural and functional damage to the hippocampal neurovascular unit in diabetes-related depression.
β-Caryophyllene, a CB2-Receptor-Selective Phytocannabinoid, Suppresses Mechanical Allodynia in a Mouse Model of Antiretroviral-Induced Neuropathic Pain.
Protective effects of ghrelin on brain mitochondria after cardiac arrest and resuscitation.
CXCL12 induces migration of Schwann cells via p38 MAPK and autocrine of CXCL12 by the CXCR4 receptor.
Polycaprolactone/polysialic acid hybrid, multifunctional nanofiber scaffolds for treatment of spinal cord injury.
The neuroprotective effects and probable mechanisms of Ligustilide and its degradative products on intracerebral hemorrhage in mice.
Shunaoxin dropping pill, a Chinese herb compound preparation, attenuates memory impairment in d-galactose-induced aging mice.
A Small Organic Compound Mimicking the L1 Cell Adhesion Molecule Promotes Functional Recovery after Spinal Cord Injury in Zebrafish.
Neuroprotective Effects of Pycnogenol Against Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury in Primary Rat Astrocytes via NF-κB and ERK1/2 MAPK Pathways.
Promoting oligodendroglial-oriented differentiation of glioma stem cell: a repurposing of quetiapine for the treatment of malignant glioma.
Association of RASgrf1 methylation with epileptic seizures.
Graft of the NT-3 persistent delivery gelatin sponge scaffold promotes axon regeneration, attenuates inflammation, and induces cell migration in rat and canine with spinal cord injury.
Transplantation of tissue engineering neural network and formation of neuronal relay into the transected rat spinal cord.
Differentiation of mesenchymal stem cells into neural stem cells using cerebrospinal fluid.
Neonatal intrahippocampal injection of lipopolysaccharide induces deficits in social behavior and prepulse inhibition and microglial activation in rats: Implication for a new schizophrenia animal model.
Ligustilide ameliorates neuroinflammation and brain injury in focal cerebral ischemia/reperfusion rats: involvement of inhibition of TLR4/peroxiredoxin 6 signaling.
Down-regulated expression of acid-sensing ion channel 1a in cortical lesions of patients with focal cortical dysplasia.
Altered expression of c-Abl in patients with epilepsy and in a rat model.
Morphology and functions of astrocytes cultured on water-repellent fractal tripalmitin surfaces.
Protective effects of cationic bovine serum albumin-conjugated PEGylated tanshinone IIA nanoparticles on cerebral ischemia.
The integration of NSC-derived and host neural networks after rat spinal cord transection.
Ketamine inhibits lipopolysaccharide-induced astrocytes activation by suppressing TLR4/NF-ĸB pathway.
Cancer stem cell labeling using poly(L-lysine)-modified iron oxide nanoparticles.
Cograft of neural stem cells and schwann cells overexpressing TrkC and neurotrophin-3 respectively after rat spinal cord transection.
A cascade targeting strategy for brain neuroglial cells employing nanoparticles modified with angiopep-2 peptide and EGFP-EGF1 protein.
Retinal metabolic changes in an experimental model of optic nerve transection by ex vivo 1H magnetic resonance spectroscopy.
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Electroacupuncture alleviates spatial memory deficits in METH withdrawal mice by enhancing astrocyte-mediated glutamate clearance in the dCA1.
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Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model.
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Dynamic-related protein 1 inhibitor eases epileptic seizures and can regulate equilibrative nucleoside transporter 1 expression.
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BMC neurology 2020 Sep 22;20(1):353
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α7nAchR mediates transcutaneous auricular vagus nerve stimulation-induced neuroprotection in a rat model of ischemic stroke by enhancing axonal plasticity.
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Neuroscience letters 2020 Jun 21;730:135031
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Renal and cerebral RAS interaction contributes to diabetic kidney disease.
Liu Y, Li L, Qiu M, Tan L, Zhang M, Li J, Zhu H, Jiang S, Su X, Li A
American journal of translational research 2019;11(5):2925-2939
American journal of translational research 2019;11(5):2925-2939
Biofunctionalized self-assembly of peptide amphiphile induces the differentiation of bone marrow mesenchymal stem cells into neural cells.
Ruan H, Xiao R, Jiang X, Zhao B, Wu K, Shao Z, Zhang Z, Duan H, Song Y
Molecular and cellular biochemistry 2019 Jan;450(1-2):199-207
Molecular and cellular biochemistry 2019 Jan;450(1-2):199-207
Structural and functional damage to the hippocampal neurovascular unit in diabetes-related depression.
Liu J, Wang YH, Li W, Liu L, Yang H, Meng P, Han YS
Neural regeneration research 2019 Feb;14(2):289-297
Neural regeneration research 2019 Feb;14(2):289-297
β-Caryophyllene, a CB2-Receptor-Selective Phytocannabinoid, Suppresses Mechanical Allodynia in a Mouse Model of Antiretroviral-Induced Neuropathic Pain.
Aly E, Khajah MA, Masocha W
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Molecules (Basel, Switzerland) 2019 Dec 27;25(1)
Protective effects of ghrelin on brain mitochondria after cardiac arrest and resuscitation.
Xu H, Li Y, Liu R, Wu L, Zhang C, Ding N, Ma A, Zhang J, Xie X
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Neuropeptides 2019 Aug;76:101936
CXCL12 induces migration of Schwann cells via p38 MAPK and autocrine of CXCL12 by the CXCR4 receptor.
Gao D, Sun H, Zhu J, Tang Y, Li S
International journal of clinical and experimental pathology 2018;11(6):3119-3125
International journal of clinical and experimental pathology 2018;11(6):3119-3125
Polycaprolactone/polysialic acid hybrid, multifunctional nanofiber scaffolds for treatment of spinal cord injury.
Zhang S, Wang XJ, Li WS, Xu XL, Hu JB, Kang XQ, Qi J, Ying XY, You J, Du YZ
Acta biomaterialia 2018 Sep 1;77:15-27
Acta biomaterialia 2018 Sep 1;77:15-27
The neuroprotective effects and probable mechanisms of Ligustilide and its degradative products on intracerebral hemorrhage in mice.
Han L, Liu DL, Zeng QK, Shi MQ, Zhao LX, He Q, Kuang X, Du JR
International immunopharmacology 2018 Oct;63:43-57
International immunopharmacology 2018 Oct;63:43-57
Shunaoxin dropping pill, a Chinese herb compound preparation, attenuates memory impairment in d-galactose-induced aging mice.
Zhou H, Qu Z, Zhang J, Jiang B, Liu C, Gao W
RSC advances 2018 Mar 13;8(19):10163-10171
RSC advances 2018 Mar 13;8(19):10163-10171
A Small Organic Compound Mimicking the L1 Cell Adhesion Molecule Promotes Functional Recovery after Spinal Cord Injury in Zebrafish.
Sahu S, Zhang Z, Li R, Hu J, Shen H, Loers G, Shen Y, Schachner M
Molecular neurobiology 2018 Jan;55(1):859-878
Molecular neurobiology 2018 Jan;55(1):859-878
Neuroprotective Effects of Pycnogenol Against Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury in Primary Rat Astrocytes via NF-κB and ERK1/2 MAPK Pathways.
Xia R, Ji C, Zhang L
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(3):987-998
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(3):987-998
Promoting oligodendroglial-oriented differentiation of glioma stem cell: a repurposing of quetiapine for the treatment of malignant glioma.
Wang Y, Huang N, Li H, Liu S, Chen X, Yu S, Wu N, Bian XW, Shen HY, Li C, Xiao L
Oncotarget 2017 Jun 6;8(23):37511-37524
Oncotarget 2017 Jun 6;8(23):37511-37524
Association of RASgrf1 methylation with epileptic seizures.
Chen X, Peng X, Wang L, Fu X, Zhou JX, Zhu B, Luo J, Wang X, Xiao Z
Oncotarget 2017 Jul 11;8(28):46286-46297
Oncotarget 2017 Jul 11;8(28):46286-46297
Graft of the NT-3 persistent delivery gelatin sponge scaffold promotes axon regeneration, attenuates inflammation, and induces cell migration in rat and canine with spinal cord injury.
Li G, Che MT, Zhang K, Qin LN, Zhang YT, Chen RQ, Rong LM, Liu S, Ding Y, Shen HY, Long SM, Wu JL, Ling EA, Zeng YS
Biomaterials 2016 Mar;83:233-48
Biomaterials 2016 Mar;83:233-48
Transplantation of tissue engineering neural network and formation of neuronal relay into the transected rat spinal cord.
Lai BQ, Che MT, Du BL, Zeng X, Ma YH, Feng B, Qiu XC, Zhang K, Liu S, Shen HY, Wu JL, Ling EA, Zeng YS
Biomaterials 2016 Dec;109:40-54
Biomaterials 2016 Dec;109:40-54
Differentiation of mesenchymal stem cells into neural stem cells using cerebrospinal fluid.
Ge W, Ren C, Duan X, Geng D, Zhang C, Liu X, Chen H, Wan M, Geng R
Cell biochemistry and biophysics 2015 Jan;71(1):449-55
Cell biochemistry and biophysics 2015 Jan;71(1):449-55
Neonatal intrahippocampal injection of lipopolysaccharide induces deficits in social behavior and prepulse inhibition and microglial activation in rats: Implication for a new schizophrenia animal model.
Zhu F, Zhang L, Ding YQ, Zhao J, Zheng Y
Brain, behavior, and immunity 2014 May;38:166-74
Brain, behavior, and immunity 2014 May;38:166-74
Ligustilide ameliorates neuroinflammation and brain injury in focal cerebral ischemia/reperfusion rats: involvement of inhibition of TLR4/peroxiredoxin 6 signaling.
Kuang X, Wang LF, Yu L, Li YJ, Wang YN, He Q, Chen C, Du JR
Free radical biology & medicine 2014 Jun;71:165-175
Free radical biology & medicine 2014 Jun;71:165-175
Down-regulated expression of acid-sensing ion channel 1a in cortical lesions of patients with focal cortical dysplasia.
Guo W, Chen X, He JJ, Wei YJ, Zang ZL, Liu SY, Yang H, Zhang CQ
Journal of molecular neuroscience : MN 2014 Jun;53(2):176-82
Journal of molecular neuroscience : MN 2014 Jun;53(2):176-82
Altered expression of c-Abl in patients with epilepsy and in a rat model.
Chen L, Wang Z, Tang B, Fang M, Li J, Chen G, Wang X
Synapse (New York, N.Y.) 2014 Jul;68(7):306-16
Synapse (New York, N.Y.) 2014 Jul;68(7):306-16
Morphology and functions of astrocytes cultured on water-repellent fractal tripalmitin surfaces.
Hu WW, Wang Z, Zhang SS, Jiang L, Zhang J, Zhang X, Lei QF, Park HJ, Fang WJ, Chen Z
Biomaterials 2014 Aug;35(26):7386-97
Biomaterials 2014 Aug;35(26):7386-97
Protective effects of cationic bovine serum albumin-conjugated PEGylated tanshinone IIA nanoparticles on cerebral ischemia.
Liu X, An C, Jin P, Liu X, Wang L
Biomaterials 2013 Jan;34(3):817-30
Biomaterials 2013 Jan;34(3):817-30
The integration of NSC-derived and host neural networks after rat spinal cord transection.
Lai BQ, Wang JM, Duan JJ, Chen YF, Gu HY, Ling EA, Wu JL, Zeng YS
Biomaterials 2013 Apr;34(12):2888-901
Biomaterials 2013 Apr;34(12):2888-901
Ketamine inhibits lipopolysaccharide-induced astrocytes activation by suppressing TLR4/NF-ĸB pathway.
Wu Y, Li W, Zhou C, Lu F, Gao T, Liu Y, Cao J, Zhang Y, Zhang Y, Zhou C
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2012;30(3):609-17
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2012;30(3):609-17
Cancer stem cell labeling using poly(L-lysine)-modified iron oxide nanoparticles.
Wang X, Wei F, Liu A, Wang L, Wang JC, Ren L, Liu W, Tu Q, Li L, Wang J
Biomaterials 2012 May;33(14):3719-32
Biomaterials 2012 May;33(14):3719-32
Cograft of neural stem cells and schwann cells overexpressing TrkC and neurotrophin-3 respectively after rat spinal cord transection.
Wang JM, Zeng YS, Wu JL, Li Y, Teng YD
Biomaterials 2011 Oct;32(30):7454-68
Biomaterials 2011 Oct;32(30):7454-68
A cascade targeting strategy for brain neuroglial cells employing nanoparticles modified with angiopep-2 peptide and EGFP-EGF1 protein.
Huile G, Shuaiqi P, Zhi Y, Shijie C, Chen C, Xinguo J, Shun S, Zhiqing P, Yu H
Biomaterials 2011 Nov;32(33):8669-75
Biomaterials 2011 Nov;32(33):8669-75
Retinal metabolic changes in an experimental model of optic nerve transection by ex vivo 1H magnetic resonance spectroscopy.
Li S, Huang M, Wang X, Wang X, Chen F, Lei H, Jiang F
Neurochemical research 2011 Dec;36(12):2427-33
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- Main image
- Experimental details
- IHC analysis of GFAP using anti-GFAP antibody (PB9082). GFAP was detected in paraffin-embedded section of Mouse Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image